Molecular and phenotypic characterization of Escherichia coli from calves in an important meat-producing region in Brazil.

Autor:	Tutija JF; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. juliane_tutija@hotmail.com., Ramos CA; Federal University of Mato Grosso do Sul, Campo Grande, Brazil., Lemos RA; Federal University of Mato Grosso do Sul, Campo Grande, Brazil., Santos AA; Federal University of Mato Grosso do Sul, Campo Grande, Brazil., Reckziegel GH; Federal University of Mato Grosso do Sul, Campo Grande, Brazil., Freitas MG; Federal University of Mato Grosso do Sul, Campo Grande, Brazil., Leal CR; Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of infection in developing countries [J Infect Dev Ctries] 2022 Jun 30; Vol. 16 (6), pp. 1030-1036. Date of Electronic Publication: 2022 Jun 30.
DOI:	10.3855/jidc.13377
Abstrakt:	Introduction: Escherichia coli strains that lead to enteritis are considered an important cause of diarrhea in calves. For correct identification, these microorganisms must be differentiated from non-pathogenic members of the intestinal microbiota. The aim of the present work was to characterize E. coli isolates in calves regarding the presence of virulence genes that cause enteritis and evaluate the sensitivity of the isolates to different antimicrobials. Methodology: One hundred forty-nine samples from beef cattle and 27 samples from dairy cattle were evaluated. All samples were submitted to microbiological identification and the disk diffusion antibiogram test. The polymerase chain reaction method was used to detect virulence genes. Results: A hundred seventy-six samples were biochemically identified as E. coli and antibiograms were determined. The samples were then submitted to PCR; 35 were positive for the eae gene (19.88%), 135 (76.70%) for the stx1 gene, 62 (35.22%) for the stx2 gene, 159 (90.34%) for the sta gene and 35 (19.88%) for the ltII gene. No samples were positive for the cnf gene. Based on these results, the E. coli isolates were classified into pathotypes: enteropathogenic (n = 3), enterohemorrhagic (n = 32), Shiga toxin-producing (n = 122) and enterotoxigenic (n = 163). The antimicrobial sensitivity tests revealed that 77.2% of the isolates were resistant to three or more pharmacological groups, characterizing these isolates as multidrug resistant. Conclusions: Enterotoxigenic E. coli was the predominant pathotype. Moreover, the prevalence of multidrug-resistant isolates was very high, accounting for the vast majority of isolates. Competing Interests: No Conflict of Interest is declared (Copyright (c) 2022 Juliane F Tutija, Carlos AN Ramos, Ricardo AA Lemos, Amanda AL Santos, Guilherme H Reckziegel, Mariana G Freitas, Cassia RB Leal.)
Databáze:	MEDLINE
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