Amazonian Guarana- and Açai-Conjugated Extracts Improve Scratched Fibroblast Healing and Eisenia fetida Surgical Tail Amputation by Modulating Oxidative Metabolism.

Autor: Felin FD; Mestrado Profissional em Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Maia-Ribeiro EA; Universidade do Estado do Amazonas-Fundação Universidade Aberta da Terceira Idade (FUNATI), Manaus, AM, Brazil 69029-000., Felin CD; Pontíficia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil 90619-900., Bonotto NAC; Programa de Pós-Graduação em Gerontologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Turra BO; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Roggia I; Programa de Pós-Graduação em Gerontologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Azzolin VF; Programa de Pós-Graduação em Gerontologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Teixeira CF; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Mastella MH; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., de Freitas CR; Laboratório de Biogenômica, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Greijanim J; Laboratório de Biogenômica, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Santos D; Departamento de Química, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Flores EMM; Laboratório de Biogenômica, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Barbisan F; Programa de Pós-Graduação em Gerontologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000.; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000.; Departamento de Patologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Cruz IBM; Programa de Pós-Graduação em Gerontologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000.; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000., Ribeiro TA; Mestrado Profissional em Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil 97105-9000.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2022 Jun 26; Vol. 2022, pp. 3094362. Date of Electronic Publication: 2022 Jun 26 (Print Publication: 2022).
DOI: 10.1155/2022/3094362
Abstrakt: Background: Previous studies have suggested that guarana ( Paullinia cupana ) and açai ( Euterpe oleracea ) have antioxidant, anti-inflammatory, and proliferative properties, indicating their potential therapeutic action in wound healing. We produced a conjugated guarana-açai (GA) extract and tested its healing action on earthworms ( Eisenia fetida ) subjected to tail amputation by surgical incision.
Methods: Extract from roasted guarana seeds and fresh açai seed berries was produced. The antioxidant and genoprotective capacity of GA extract was tested. The concentration with the most remarkable healing potential was used in subsequent tests. The last three posterior segments of the clitellate earthworm tail reared under standardized conditions were surgically amputated. Next, topical PBS or GA extract was applied to the surgical wound. The rate of cell migration and tissue regeneration at the local wound site was histologically evaluated after the procedure. Expression of the SOX4 gene that acts in epithelial-to-mesenchymal transition was determined by RT-qPCR.
Results: Sixteen bioactive molecules, including some previously described substances, were identified. All tested concentrations exhibited antioxidant and genoprotective effects. The GA extract accelerated the healing processes as observed through macroscopic and histological analyses and increased expression of SOX4.
Conclusion: The GA extract has a potential role in the healing of surgically induced wounds.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.
(Copyright © 2022 Fellipe D. Felin et al.)
Databáze: MEDLINE
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