Genetic diversity of enterotoxigenic Bacillus cereus strains in coriander in southwestern Mexico.
| Autor: | Castulo-Arcos DA; Laboratorio de Investigación en Patometabolismo Microbiano/Facultad de Ciencias Químico Biológicas, Universidad Autonoma de Guerrero, Chilpancingo, Guerrero, Mexico., Adame-Gómez R; Laboratorio de Investigación en Patometabolismo Microbiano/Facultad de Ciencias Químico Biológicas, Universidad Autonoma de Guerrero, Chilpancingo, Guerrero, Mexico., Castro-Alarcón N; Laboratorio de Investigación en Microbiología/Facultad de Ciencias Químico Biológicas, Universidad Autonoma de Guerrero, Chilpancingo, Guerrero, México., Galán-Luciano A; Laboratorio de Investigación en Patometabolismo Microbiano/Facultad de Ciencias Químico Biológicas, Universidad Autonoma de Guerrero, Chilpancingo, Guerrero, Mexico., Santiago Dionisio MC; Laboratorio de Investigación en Análisis Microbiológicos/Facultad de Ciencias Químico Biológicas, Universidad Autonoma de Guerrero, Chilpancingo, Guerrero, México., Leyva-Vázquez MA; Laboratorio de Investigación en Biomedicina Molecular/Facultad de Ciencias Químico Biológicas, Universidad Autonoma de Guerrero, Chilpancingo, Guerrero, México., Perez-Olais JH; Laboratorio de Biología Celular/Unidad Cuajimalpa, Universidad Autonoma Metropolitana, Ciudad de México, Ciudad de México, México., Toribio-Jiménez J; Laboratorio de Investigacion en Microbiologia Molecular y Biotecnologia Ambiental/Facultad de Ciencias Químico Biológicas, Universidad Autonoma de Guerrero, Chilpancingo, Guerrero, Mexico., Ramirez-Peralta A; Laboratorio de Investigación en Patometabolismo Microbiano/Facultad de Ciencias Químico Biológicas, Universidad Autonoma de Guerrero, Chilpancingo, Guerrero, Mexico. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | PeerJ [PeerJ] 2022 Jul 01; Vol. 10, pp. e13667. Date of Electronic Publication: 2022 Jul 01 (Print Publication: 2022). |
| DOI: | 10.7717/peerj.13667 |
| Abstrakt: | Background: Coriander, like other leafy green vegetables, is available all year round and is commonly consumed raw in Mexico as in other countries in the preparation of street or homemade food. Bacillus cereus ( B. cereus ) is a microorganism that can reach coriander because it is usually found in the soil and in some regions the vegetables are irrigated with polluted water. Therefore, the aim of this study was to determinate the presence of B. cereus in coriander used for human consumption in southwestern Mexico and determine the toxigenic profile, biofilm production, genes associated with the production of biofilms, sporulation rates, enzymatic profile, psychotropic properties, and genetic diversity of B. cereus . Methods: Fresh coriander samples were collected from several vegetable retailers in different markets, microbiological analysis was performed. Molecular identification, genes related to the production of biofilm, and toxin gene profiling of B. cereus isolates were determined by PCR. The biofilm formation was measured by performing a crystal violet assay. The genetic diversity of B. cereus strains was determined by PCR of repetitive elements using oligonucleotide (GTG) 5. Results: We found a frequency of B. cereus in vegetables was 20% (13/65). In this study, no strains with genes for the HBL toxin were found. In the case of genes related to biofilms, the frequency was low for sipW [5.8%, (1/17)] and tasA [11.7%, (2/17)]. B. cereus strains produce a low amount of biofilm with sporulation rates around 80%. As for genetic diversity, we observed that strains isolated from the same market, but different vegetable retailers are grouped into clusters. In the coriander marketed in southwestern Mexico, were found B. cereus strains with genes associated with the production of diarrheal toxins. Together, these results show actual information about the state of art of B. cereus strains circulating in the southwestern of Mexico. Competing Interests: The authors declare that they have no competing interests. (© 2022 Castulo-Arcos et al.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|