A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?

Autor:	Lampejo AO; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States., Hu NW; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States., Lucas D; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States., Lomel BM; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States., Nguyen CM; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States., Dominguez CC; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States., Ren B; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States., Huang Y; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States., Murfee WL; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2022 Jun 20; Vol. 10, pp. 912073. Date of Electronic Publication: 2022 Jun 20 (Print Publication: 2022).
DOI:	10.3389/fbioe.2022.912073
Abstrakt:	The gap between in vitro and in vivo assays has inspired biomimetic model development. Tissue engineered models that attempt to mimic the complexity of microvascular networks have emerged as tools for investigating cell-cell and cell-environment interactions that may be not easily viewed in vivo . A key challenge in model development, however, is determining how to recreate the multi-cell/system functional complexity of a real network environment that integrates endothelial cells, smooth muscle cells, vascular pericytes, lymphatics, nerves, fluid flow, extracellular matrix, and inflammatory cells. The objective of this mini-review is to overview the recent evolution of popular biomimetic modeling approaches for investigating microvascular dynamics. A specific focus will highlight the engineering design requirements needed to match physiological function and the potential for top-down tissue culture methods that maintain complexity. Overall, examples of physiological validation, basic science discoveries, and therapeutic evaluation studies will emphasize the value of tissue culture models and biomimetic model development approaches that fill the gap between in vitro and in vivo assays and guide how vascular biologists and physiologists might think about the microcirculation. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Lampejo, Hu, Lucas, Lomel, Nguyen, Dominguez, Ren, Huang and Murfee.)
Databáze:	MEDLINE
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