Discovery of archaeal fusexins homologous to eukaryotic HAP2/GCS1 gamete fusion proteins.

Autor: Moi D; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Buenos Aires, Argentina.; Department of Computational Biology, University of Lausanne, Lausanne, Switzerland.; Swiss Institute of Bioinformatics, Lausanne, Switzerland., Nishio S; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden., Li X; Department of Biology, Technion- Israel Institute of Technology, Haifa, Israel., Valansi C; Department of Biology, Technion- Israel Institute of Technology, Haifa, Israel., Langleib M; Unidad de Genómica Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.; Unidad de Bioinformática, Institut Pasteur de Montevideo, Montevideo, Uruguay., Brukman NG; Department of Biology, Technion- Israel Institute of Technology, Haifa, Israel., Flyak K; Department of Biology, Technion- Israel Institute of Technology, Haifa, Israel., Dessimoz C; Department of Computational Biology, University of Lausanne, Lausanne, Switzerland.; Swiss Institute of Bioinformatics, Lausanne, Switzerland.; Department of Genetics, Evolution and Environment, Centre for Life's Origins and Evolution, University College London, London, UK.; Department of Computer Science, University College London, London, UK., de Sanctis D; ESRF-The European Synchrotron, Grenoble, France., Tunyasuvunakool K; DeepMind, London, UK., Jumper J; DeepMind, London, UK., Graña M; Unidad de Bioinformática, Institut Pasteur de Montevideo, Montevideo, Uruguay. mgrana@pasteur.edu.uy., Romero H; Unidad de Genómica Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. eletor@fcien.edu.uy.; Centro Universitario Regional Este - CURE, Centro Interdisciplinario de Ciencia de Datos y Aprendizaje Automático - CICADA, Universidad de la República, Montevideo, Uruguay. eletor@fcien.edu.uy., Aguilar PS; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Buenos Aires, Argentina. paguilar@iib.unsam.edu.ar.; Instituto de Investigaciones Biotecnológicas Universidad Nacional de San Martín (IIB-CONICET), San Martín, Buenos Aires, Argentina. paguilar@iib.unsam.edu.ar., Jovine L; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden. luca.jovine@ki.se., Podbilewicz B; Department of Biology, Technion- Israel Institute of Technology, Haifa, Israel. podbilew@technion.ac.il.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Jul 06; Vol. 13 (1), pp. 3880. Date of Electronic Publication: 2022 Jul 06.
DOI: 10.1038/s41467-022-31564-1
Abstrakt: Sexual reproduction consists of genome reduction by meiosis and subsequent gamete fusion. The presence of genes homologous to eukaryotic meiotic genes in archaea and bacteria suggests that DNA repair mechanisms evolved towards meiotic recombination. However, fusogenic proteins resembling those found in gamete fusion in eukaryotes have so far not been found in prokaryotes. Here, we identify archaeal proteins that are homologs of fusexins, a superfamily of fusogens that mediate eukaryotic gamete and somatic cell fusion, as well as virus entry. The crystal structure of a trimeric archaeal fusexin (Fusexin1 or Fsx1) reveals an archetypical fusexin architecture with unique features such as a six-helix bundle and an additional globular domain. Ectopically expressed Fusexin1 can fuse mammalian cells, and this process involves the additional globular domain and a conserved fusion loop. Furthermore, archaeal fusexin genes are found within integrated mobile elements, suggesting potential roles in cell-cell fusion and gene exchange in archaea, as well as different scenarios for the evolutionary history of fusexins.
(© 2022. The Author(s).)
Databáze: MEDLINE
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