Second biopsy for embryos with inconclusive results after preimplantation genetic testing: Impact on pregnancy outcomes.

Autor: Carles M; Service de Biologie de la Reproduction - CECOS, Hôpital Antoine Béclère, AP-HP, Université Paris Saclay, Clamart, France. Electronic address: manon.carles@live.fr., Sonigo C; Service de Médecine de la reproduction et Préservation de la Fertilité, Hôpital Antoine Béclère, AP-HP, Université Paris Saclay, Clamart, France; Inserm U1185, Faculté de médecine Paris Sud, France; Univ Paris Sud, Université Paris-Saclay, 94270 Le Kremlin-Bicêtre, France., Binois O; Service de Biologie de la Reproduction - CECOS, Hôpital Antoine Béclère, AP-HP, Université Paris Saclay, Clamart, France., Hesters L; Service de Biologie de la Reproduction - CECOS, Hôpital Antoine Béclère, AP-HP, Université Paris Saclay, Clamart, France., Steffann J; Université de Paris, Institut Imagine et Service de Génétique Moléculaire, Hôpital Necker-Enfants Malades, Paris, France., Romana S; Université de Paris, Institut Imagine et Service de Cytogénétique, Hôpital Necker-Enfants Malades, Paris, France., Frydman N; Service de Biologie de la Reproduction - CECOS, Hôpital Antoine Béclère, AP-HP, Université Paris Saclay, Clamart, France; Univ Paris Sud, Université Paris-Saclay, 94270 Le Kremlin-Bicêtre, France., Mayeur A; Service de Biologie de la Reproduction - CECOS, Hôpital Antoine Béclère, AP-HP, Université Paris Saclay, Clamart, France; Univ Paris Sud, Université Paris-Saclay, 94270 Le Kremlin-Bicêtre, France.
Jazyk: angličtina
Zdroj: Journal of gynecology obstetrics and human reproduction [J Gynecol Obstet Hum Reprod] 2022 Oct; Vol. 51 (8), pp. 102436. Date of Electronic Publication: 2022 Jul 03.
DOI: 10.1016/j.jogoh.2022.102436
Abstrakt: In this study, we aimed to evaluate the pregnancy outcomes for embryos biopsied twice at cleavage and blastocyst stage for preimplantation genetic testing (PGT). This retrospective monocentric study, conducted between January 2016 and March 2021, described all PGT results on one hand and the PGT results for undiagnosed embryos submitted to a second biopsy on the other hand. Among the 5865 embryos biopsied during the study period, 510 embryos were genetic undiagnosed after the first embryo biopsy at cleavage stage (8.7%). The rate of undiagnosed embryos was significantly higher for PGT for structural rearrangement (PGT-SR) than PGT for monogenic disease (PGT-M) (10.2% vs 7.4% respectively, p < 0.001). Thirty-three embryos were compatible with a second biopsy at blastocyst stage before being directly frozen. Among them 17 were diagnosed as healthy for the researched pathology (51.5%). At the time of our study, 11 of the 17 preserved embryos were thawed and transferred. Embryo survival at thawing was 100% and 5 pregnancies were obtained (clinical pregnancy rate of 45.5% per transfer), including 3 live births. A second biopsy for inconclusive embryos after PGT does not seem to have an impact on thaw survival and implantation rate. For couple, this strategy avoids to discard transferable embryos.
Competing Interests: Declaration of Competing Interest The authors have nothing to disclose.
(Copyright © 2022 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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