Novel laccase from Xylaria polymorpha and its efficiency in the biotransformation of pharmaceuticals: Optimization of operational conditions, comparative effect of redox-mediators and toxicity studies.

Autor: Bankole PO; Department of Pure and Applied Botany, College of Biosciences, Federal University of Agriculture P.M.B., 2240 Abeokuta, Ogun State, Nigeria; Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom. Electronic address: bankolepo@funaab.edu.ng., Omoni VT; Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom., Tennison-Omovoh CA; Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom; Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Benin, Nigeria., Adebajo SO; Department of Microbiology, College of Biosciences, Federal University of Agriculture P.M.B., 2240 Abeokuta, Ogun State, Nigeria., Mulla SI; Department of Biochemistry, School of Allied Health Sciences, REVA University, Bangalore-560064, Karnataka, India., Adekunle AA; Department of Botany, Faculty of Science, University of Lagos, Lagos State, Nigeria., Semple KT; Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2022 Sep; Vol. 217, pp. 112675. Date of Electronic Publication: 2022 Jun 30.
DOI: 10.1016/j.colsurfb.2022.112675
Abstrakt: The promising potentials of biocatalytic treatment processes in the removal of micropollutants whilst eliminating health and environmental hazards have attracted great attention in recent years. This current work investigated the biotransformation efficiency of a novel laccase from Xylaria polymorpha (XPL) in comparison with commercial laccases from Trametes versicolor (TVL) and Aspergillus sp. (ASL). XPL exhibited better oxidation performance (95.7%) on AMX than TVL (92.8%) and ASL (90.5%). Optimization of operational conditions revealed that AMX was best oxidized at pH 5, temperature (30 °C), and concentration (1.0 mg L -1 ). The investigation carried out to determine the effect of redox mediators revealed violuric acid (VLA) as the best redox mediator. The laccase stability experiments elucidated that the oxidation of AMX is time and mediator concentration dependent with ABTS exhibiting highest deactivation of XPL active sites. Two metabolic products; amoxicillin penilloic acid and 5-hydroxy-6-(4-hydroxyphenyl)- 3-(1,3-thiazolidin-2-yl)piperazin-2-one of AMX were obtained through Liquid Chromatography-Mass Spectrometry (LC-MS) analyses. The toxicity assessments carried out after oxidation of AMX by XPL showed 94% and 97% reduced toxicity on Artemia salina and Aliivibrio fischeri respectively. The study further underscored the efficiency of biocatalytic-mediator technology in the transformation of complex micropollutants into less toxic substances in an eco-friendly way.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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