Microgravity-induced stress mechanisms in human stem cell-derived cardiomyocytes.

Autor:	Acharya A; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany., Nemade H; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany., Papadopoulos S; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany., Hescheler J; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany., Neumaier F; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany., Schneider T; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany., Rajendra Prasad K; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany., Khan K; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany., Hemmersbach R; German Aerospace Center, Institute of Aerospace Medicine, Gravitational Biology, Linder Hoehe, 51147 Cologne, Germany., Gusmao EG; Institute of Pathology, University Medical Center Göttingen, 37077 Göttingen, Germany., Mizi A; Institute of Pathology, University Medical Center Göttingen, 37077 Göttingen, Germany., Papantonis A; Institute of Pathology, University Medical Center Göttingen, 37077 Göttingen, Germany.; Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany., Sachinidis A; University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany.; Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2022 Jun 11; Vol. 25 (7), pp. 104577. Date of Electronic Publication: 2022 Jun 11 (Print Publication: 2022).
DOI:	10.1016/j.isci.2022.104577
Abstrakt:	Exposure to outer space microgravity poses a risk for the development of various pathologies including cardiovascular disease. To study this, we derived cardiomyocytes (CMs) from human-induced pluripotent stem cells and exposed them to simulated microgravity (SMG). We combined different "omics" and chromosome conformation capture technologies with live-cell imaging of various transgenic lines to discover that SMG impacts on the contractile velocity and function of CMs via the induction of senescence processes. This is linked to SMG-induced changes of reactive oxygen species (ROS) generation and energy metabolism by mitochondria. Taken together, we uncover a microgravity-controlled axis causing contractile dysfunctions to CMs. Our findings can contribute to the design of preventive and therapeutic strategies against senescence-associated disease. Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).)
Databáze:	MEDLINE
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