The interaction between the Dbf4 ortholog Chiffon and Gcn5 is conserved in Dipteran insect species.

Autor: George S; Department of Biochemistry, Purdue University, West Lafayette, Indiana, USA., Blum HR; Department of Biochemistry, Purdue University, West Lafayette, Indiana, USA., Torres-Zelada EF; Department of Biochemistry, Purdue University, West Lafayette, Indiana, USA., Estep GN; Department of Biochemistry, Purdue University, West Lafayette, Indiana, USA., Hegazy YA; Department of Biochemistry, Purdue University, West Lafayette, Indiana, USA., Speer GM; Department of Biochemistry, Purdue University, West Lafayette, Indiana, USA., Weake VM; Department of Biochemistry, Purdue University, West Lafayette, Indiana, USA.; Purdue University Center for Cancer Research, Purdue University, West Lafayette, Indiana, USA.
Jazyk: angličtina
Zdroj: Insect molecular biology [Insect Mol Biol] 2022 Dec; Vol. 31 (6), pp. 734-746. Date of Electronic Publication: 2022 Jul 15.
DOI: 10.1111/imb.12800
Abstrakt: Chiffon is the sole Drosophila ortholog of Dbf4, the regulatory subunit for the cell-cycle kinase Cdc7 that initiates DNA replication. In Drosophila, the chiffon gene encodes two polypeptides with independent activities. Chiffon-A contains the conserved Dbf4 motifs and interacts with Cdc7 to form the Dbf4-dependent Kinase (DDK) complex, which is essential for a specialized form of DNA replication. In contrast, Chiffon-B binds the histone acetyltransferase Gcn5 to form the Chiffon histone acetyltransferase (CHAT) complex, which is necessary for histone H3 acetylation and viability. Previous studies have shown that the Chiffon-B region is only present within insects. However, it was unclear how widely the interaction between Chiffon-B and Gcn5 was conserved among insect species. To examine this, we performed yeast two-hybrid assays using Chiffon-B and Gcn5 from a variety of insect species and found that Chiffon-B and Gcn5 interact in Diptera species such as Australian sheep blowfly and yellow fever mosquito. Protein domain analysis identified that Chiffon-B has features of acidic transcriptional activators such as Gal4 or VP16. We propose that the CHAT complex plays a critical role in a biological process that is unique to Dipterans and could therefore be a potential target for pest control strategies.
(© 2022 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.)
Databáze: MEDLINE
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