Occurrence of bla NDM-7 and association with bla KPC-2 , bla CTX-M15 , aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil.

Autor: de Oliveira Alves W; Centro de Ciências Médicas, Universidade Federal de Pernambuco, Área de Medicina Tropical, Recife, PE, Brasil., Scavuzzi AML; Centro de Ciências Médicas, Universidade Federal de Pernambuco, Área de Medicina Tropical, Recife, PE, Brasil., Beltrão EMB; Centro de Ciências Médicas, Universidade Federal de Pernambuco, Área de Medicina Tropical, Recife, PE, Brasil., de Oliveira ÉM; Centro de Ciências Médicas, Universidade Federal de Pernambuco, Área de Medicina Tropical, Recife, PE, Brasil., Dos Santos Vasconcelos CR; Instituto Aggeu Magalhaes- IAM - FIOCRUZ-PE, Recife, Brasil., Rezende AM; Instituto Aggeu Magalhaes- IAM - FIOCRUZ-PE, Recife, Brasil., de Souza Lopes AC; Centro de Ciências Médicas, Universidade Federal de Pernambuco, Área de Medicina Tropical, Recife, PE, Brasil. ana.lopes.ufpe@gmail.com.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2022 Jul 05; Vol. 204 (8), pp. 459. Date of Electronic Publication: 2022 Jul 05.
DOI: 10.1007/s00203-022-03051-0
Abstrakt: To characterize phenotypically and genotypically an isolate of multidrug-resistant (MDR) K. pneumoniae from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated using PCR and sequencing the amplicons, and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all β-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variants bla NDM-7 associated with bla KPC-2 and the cps, wabG, fim-H, mrkD and entB virulence genes were identified. The analysis of plasmid revealed the presence of bla CTX-M15 , aac(3)-IVa, aph(3')-Ia, aph(4)-Ia, aac(6')ib-cr, mph(A) and catB3, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the bla NDM-7 gene in Recife-PE and we suggest that this variant is located in IncX3. These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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