In vitro antiplasmodial activity-directed investigation and UPLC-MS fingerprint of promising extracts and fractions from Terminalia ivorensis A. Chev. and Terminalia brownii Fresen.
| Autor: | Tali MBT; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: b.tchatat@yahoo.com., Dize D; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: darline.dize@yahoo.fr., Njonte Wouamba SC; Laboratory of Natural Products and Organic Synthesis, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon; Department of Chemistry, Higher Teacher's Training College, University of Yaoundé I, P. O. Box 47, Yaoundé, Cameroon. Electronic address: wouamba01s@yahoo.fr., Tsouh Fokou PV; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon; Department of Biochemistry, Faculty of Science, University of Bamenda, PO Box 39, Bambili, Bamenda, Cameroon. Electronic address: ptsouh@gmail.com., Keumoe R; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: rkeumoe@yahoo.fr., Ngansop CN; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: njampacyrille2@yahoo.com., Nguembou Njionhou MS; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: m.nguembou@yahoo.fr., Jiatsa Mbouna CD; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: cedrickjiatsa@yahoo.com., Yamthe Tchokouaha LR; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon; Institute for Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, P.O. Box 6163, Yaoundé, Cameroon. Electronic address: yamthe_lauve@yahoo.fr., Maharaj V; Department of Chemistry, University of Pretoria, Hatfield Campus, Hatfield, 0028, South Africa. Electronic address: vinesh.maharaj@up.ac.za., Khorommbi NK; Department of Chemistry, University of Pretoria, Hatfield Campus, Hatfield, 0028, South Africa. Electronic address: khorombink@gmail.com., Naidoo-Maharaj D; Department of Chemistry, University of Pretoria, Hatfield Campus, Hatfield, 0028, South Africa; Agricultural Research Council-Vegetables, Industrial and Medicinal Plants, Private Bag X293, Pretoria, 0001, South Africa. Electronic address: dashnienaidoo@gmail.com., Tchouankeu JC; Laboratory of Natural Products and Organic Synthesis, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: jctchouank@yahoo.com., Boyom FF; Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: fabrice.boyom@fulbrightmail.org. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of ethnopharmacology [J Ethnopharmacol] 2022 Oct 05; Vol. 296, pp. 115512. Date of Electronic Publication: 2022 Jul 01. |
| DOI: | 10.1016/j.jep.2022.115512 |
| Abstrakt: | Ethnopharmacological Significance: Medicinal plants from the Terminalia genus are widely used as remedies against many infectious diseases, including malaria. As such, Terminalia ivorensis A. Chev. and Terminalia brownii Fresen. are famous due to their usefulness in traditional medicines to treat malaria and yellow fever. However, further information is needed on the extent of anti-Plasmodium potency of extracts and fractions from these plants and their phytochemical profile. Aim of the Study: This study was designed to investigate the in vitro antiplasmodial activity and to determine the chemical profile of promising extracts and fractions from T. ivorensis and T. brownii stem bark. Materials and Methods: Crude aqueous, ethanolic, methanolic, hydroethanolic and ethyl acetate extracts were prepared by maceration from the stem barks of T. brownii and T. ivorensis. They were subsequently tested against chloroquine-sensitive (Pf3D7) and multidrug-resistant (PfDd2) strains of P. falciparum using the parasite lactate dehydrogenase (PfLDH) assay. Extracts showing very good activity on both plasmodial strains were further fractionated using column chromatography guided by evidence of antiplasmodial activity. All bioactive extracts and fractions were screened for their cytotoxicity on Vero and Raw cell lines using the resazurin-based assay and on erythrocytes using the hemolysis assay. The phytochemical profiles of selected potent extracts and fractions were determined by UPLC-QTOF-MS analysis. Results: Of the ten extracts obtained from both plant species, nine showed inhibitory activity against both P. falciparum strains (Pf3D7 and PfDd2), with median inhibitory concentration (IC Conclusions: The extracts and fractions from T. ivorensis and T. brownii showed very good antiplasmodial activity, thus supporting the traditional use of the two plants in the treatment of malaria. Chemical profiling of the extracts and fractions led to the identification of chemical markers and the known antimalarial compound ellagic acid. Further isolation and testing of other pure compounds from the active fractions could lead to the identification of potent antiplasmodial compounds. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| Databáze: | MEDLINE |
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