Detection of SARS-CoV-2 RNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) on Self-Collected Nasal Swab Compared With Professionally Collected Nasopharyngeal Swab.

Autor:	Mannan N; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Raihan R; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Parvin US; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Fazle Akbar SM; Department of Gastroenterology and Hepatology, Ehime University Graduate School of Medicine, Toon City, JPN., Reza MS; Molecular Diagnostics Laboratory, Bangabandhu Sheikh Mujib Medical College, Faridpur, BGD., Islam S; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Kundu J; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Noman AA; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Fakhruddin M; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Billaha M; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Anik M; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Hasan T; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Tudu N; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Rahim A; Department of Microbiology, US-Bangla Medical College and Hospital, Narayanganj, BGD., Mim F; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka, BGD., Rahman Khan MJ; Department of Microbiology, Shaheed Suhrawardy Medical College, Dhaka, BGD.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2022 Jun 03; Vol. 14 (6), pp. e25618. Date of Electronic Publication: 2022 Jun 03 (Print Publication: 2022).
DOI:	10.7759/cureus.25618
Abstrakt:	Background: Self-collection of nasal swabs for the detection of SARS-CoV-2 RNA by reverse transcription-polymerase chain reaction (RT-PCR) would considerably increase the testing capability and decrease the risk of transmission among healthcare workers (HCW) and the use of personal protective equipment (PPE). Objectives: This study aimed to evaluate the performance of self-collected nasal swabs compared with professionally collected nasopharyngeal (NP) swabs for detection of SARS-CoV-2 RNA by RT-PCR. Materials and Methods: We performed a cross-sectional study where the suspected cases of coronavirus disease 2019 (COVID-19) were instructed about the self-collection of nasal swabs from their mid-turbinate. The results were compared to a nasopharyngeal swab collected by a trained healthcare worker in the same patient at the same sitting. Results: We enrolled 100 participants, of which, 69 (69%) were male and 31 (31%) were female. The median age of the study participant was 36 years. Of the participants, 58 (58%) were symptomatic, and the commonest clinical presentation was cough, which was present in 42 (42%) participants. Out of 100 samples, 31 (31%) professionally collected nasopharyngeal swabs and 28 (28%) self-collected nasal swabs were positive for SARS-CoV-2 by RT-PCR. Out of 31 professionally collected positive samples, three samples were negative in self-collection. Out of 28 self-collected positive samples, no sample was negative in the professional collection. The sensitivity and specificity of self-collected nasal swabs compared to professionally collected nasopharyngeal swabs were 90.32% and 100.00%, respectively. The sensitivity of self-collected nasal was 100% when the cycle threshold (Ct) value of the professionally collected NP swab was less than 30. Conclusion: Our study showed that self-collected nasal swabs' sensitivities were similar to professionally collected NP swabs with a high viral load (low Ct value). Hence, this method could be used when the patient is symptomatic and come to the health providers in the early stage of COVID-19 illness. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2022, Mannan et al.)
Databáze:	MEDLINE
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