Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation.

Autor:	Louro AF; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica Aónio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal., Virgolini N; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica Aónio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal., Paiva MA; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica Aónio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal., Isidro IA; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica Aónio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal., Alves PM; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica Aónio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal., Gomes-Alves P; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica Aónio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal., Serra M; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica Aónio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2022 Jun 16; Vol. 13, pp. 926528. Date of Electronic Publication: 2022 Jun 16 (Print Publication: 2022).
DOI:	10.3389/fphys.2022.926528
Abstrakt:	Extracellular Vesicles (EV) play a critical role in the regulation of regenerative processes in wounded tissues by mediating cell-to-cell communication. Multiple RNA species have been identified in EV, although their function still lacks understanding. We previously characterized the miRNA content of EV secreted over hiPSC-cardiomyocyte differentiation and found a distinct miRNA expression in hiPSC-EV driving its in vitro bioactivity. In this work, we investigated the piRNA profiles of EV derived from key stages of the hiPSC-CM differentiation and maturation, i.e., from hiPSC (hiPSC-EV), cardiac progenitors (CPC-EV), immature (CMi-EV), and mature (CMm-EV) cardiomyocytes, demonstrating that EV-piRNA expression differs greatly from the miRNA profiles we previously identified. Only four piRNA were significantly deregulated in EV, one in hiPSC-EV, and three in CPC-EV, as determined by differential expression analysis on small RNA-seq data. Our results provide a valuable source of information for further studies aiming at defining the role of piRNA in the bioactivity and therapeutic potential of EV. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Louro, Virgolini, Paiva, Isidro, Alves, Gomes-Alves and Serra.)
Databáze:	MEDLINE
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