Rapid detection of an Ebola biomarker with optical microring resonators.

Autor:	Qavi AJ; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA., Meserve K; Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA., Aman MJ; Integrated Biotherapeutics, Rockville, MD 20850, USA., Vu H; Integrated Biotherapeutics, Rockville, MD 20850, USA., Zeitlin L; Mapp Biopharmaceutical, Inc., San Diego, CA 92121, USA., Dye JM; United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA., Froude JW; United States Army Nuclear and Countering Weapons of Mass Destruction Agency, Fort Belvoir, VA 22060, USA., Leung DW; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA., Yang L; Department of Electrical & Systems Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA., Holtsberg FW; Integrated Biotherapeutics, Rockville, MD 20850, USA., Bailey RC; Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA., Amarasinghe GK; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Jazyk:	angličtina
Zdroj:	Cell reports methods [Cell Rep Methods] 2022 Jun 08; Vol. 2 (6), pp. 100234. Date of Electronic Publication: 2022 Jun 08 (Print Publication: 2022).
DOI:	10.1016/j.crmeth.2022.100234
Abstrakt:	Ebola virus (EBOV) is a highly infectious pathogen, with a case mortality rate as high as 89%. Rapid therapeutic treatments and supportive measures can drastically improve patient outcome; however, the symptoms of EBOV disease (EVD) lack specificity from other endemic diseases. Given the high mortality and significant symptom overlap, there is a critical need for sensitive, rapid diagnostics for EVD. Facile diagnosis of EVD remains a challenge. Here, we describe a rapid and sensitive diagnostic for EVD through microring resonator sensors in conjunction with a unique biomarker of EBOV infection, soluble glycoprotein (sGP). Microring resonator sensors detected sGP in under 40 min with a limit of detection (LOD) as low as 1.00 ng/mL in serum. Furthermore, we validated our assay with the detection of sGP in serum from EBOV-infected non-human primates. Our results demonstrate the utility of a high-sensitivity diagnostic platform for detection of sGP for diagnosis of EVD. Competing Interests: R.C.B. has minor financial interest in Genalyte, Inc. M.J.A., H.V., and F.W.H. are employees of Integrated BioTherapeutics, Inc. L.Z. is co-owner of Mapp Biopharmaceutical, Inc. (© 2022 The Authors.)
Databáze:	MEDLINE
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