Clustered Regularly Interspaced Short Palindromic Repeats Genotyping of Multidrug-Resistant Salmonella Heidelberg Strains Isolated From the Poultry Production Chain Across Brazil.
| Autor: | Monte DFM; Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, Food Research Center, University of São Paulo, São Paulo, Brazil.; Laboratory of Avian Pathology, Department of Pathology, Theriogenology, and One Health, São Paulo State University (FCAV-Unesp), São Paulo, Brazil., Nethery MA; Genomic Sciences Graduate Program, North Carolina State University, Raleigh, NC, United States.; Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC, United States., Berman H; Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States., Keelara S; Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States., Lincopan N; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil., Fedorka-Cray PJ; Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States., Barrangou R; Genomic Sciences Graduate Program, North Carolina State University, Raleigh, NC, United States.; Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC, United States., Landgraf M; Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, Food Research Center, University of São Paulo, São Paulo, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2022 Jun 17; Vol. 13, pp. 867278. Date of Electronic Publication: 2022 Jun 17 (Print Publication: 2022). |
| DOI: | 10.3389/fmicb.2022.867278 |
| Abstrakt: | Salmonella enterica subsp. enterica serovar Heidelberg has been associated with a broad host range, such as poultry, dairy calves, swine, wild birds, environment, and humans. The continuous evolution of S . Heidelberg raises a public health concern since there is a global dispersal of lineages harboring a wide resistome and virulome on a global scale. Here, we characterized the resistome, phylogenetic structure and clustered regularly interspaced short palindromic repeats (CRISPR) array composition of 81 S . Heidelberg strains isolated from broiler farms ( n = 16), transport and lairage ( n = 5), slaughterhouse ( n = 22), and retail market ( n = 38) of the poultry production chain in Brazil, between 2015 and 2016 using high-resolution approaches including whole-genome sequencing (WGS) and WGS-derived CRISPR genotyping. More than 91% of the S . Heidelberg strains were multidrug-resistant. The total antimicrobial resistance (AMR) gene abundances did not vary significantly across regions and sources suggesting the widespread distribution of antibiotic-resistant strains from farm to market. The highest AMR gene abundance was observed for fosA7 , aac(6')-Iaa , sul2 , tet(A) , gyrA , and parC for 100% of the isolates, followed by 88.8% for bla Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Monte, Nethery, Berman, Keelara, Lincopan, Fedorka-Cray, Barrangou and Landgraf.) |
| Databáze: | MEDLINE |
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