New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus.

Autor:	Norpi ASM; Centre for Drug Delivery Technology, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.; Faculty Pharmacy and Health Sciences, Universiti Kuala Lumpur, Royal College of Medicine Perak, Perak 30450, Malaysia., Nordin ML; Centre for Drug Delivery Technology, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.; Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kelantan 16100, Malaysia., Ahmad N; Centre for Drug Delivery Technology, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia., Katas H; Centre for Drug Delivery Technology, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia., Fuaad AAA; Department of Chemistry, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia., Sukri A; Universiti Teknologi Mara (UiTM), Bandar Puncak Alam, Integrative Pharmacogenomics Institute (iPROMISE), Selangor 43200, Malaysia., Marasini N; School of Biomedical Sciences, The University of Queensland, St. Lucia QLD 4072, Australia., Azmi F; Centre for Drug Delivery Technology, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.
Jazyk:	angličtina
Zdroj:	Asian journal of pharmaceutical sciences [Asian J Pharm Sci] 2022 May; Vol. 17 (3), pp. 435-446. Date of Electronic Publication: 2022 Apr 30.
DOI:	10.1016/j.ajps.2022.04.002
Abstrakt:	An effective vaccine against group A streptococcus (GAS) is highly desirable for definitive control of GAS infections. In the present study, two variants of amphiphilic chitosan nanoparticles-based GAS vaccines were developed. The vaccines were primarily composed of encapsulated KLH protein (a source of T helper cell epitopes) and lipidated M-protein derived B cell peptide epitope (lipoJ14) within the amphiphilic structure of nanoparticles. The only difference between them was one of the nanoparticles vaccines received additional surface coating with poly (I:C). The formulated vaccines exhibited nanosized particles within the range of 220-240 nm. Cellular uptake study showed that nanoparticles vaccine without additional poly (I:C) coating has greater uptake by dendritic cells and macrophages compared to nanoparticles vaccine that was functionalized with poly (I:C). Both vaccines were found to be safe in mice and showed negligible cytotoxicity against HEK293 cells. Upon immunization in mice, both nanoparticle vaccines produced high antigen-specific antibodies titres that were regulated by a balanced Th1 and Th2 response compared to physical mixture. These antibodies elicited high opsonic activity against the tested GAS strains. Overall, our data demonstrated that amphiphilic chitosan nanoparticles platform induced a potent immune response even without additional inclusion of poly (I:C). Competing Interests: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article. (© 2022 Shenyang Pharmaceutical University. Published by Elsevier B.V.)
Databáze:	MEDLINE
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