Multi-residue analytical method for detecting pesticides, veterinary drugs, and mycotoxins in feed using liquid- and gas chromatography coupled with mass spectrometry.

Autor: Na TW; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea., Seo HJ; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea., Jang SN; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea., Kim H; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea., Yun H; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea., Kim H; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea., Ahn J; National Agricultural Products Quality Management Service, 1430, Cheonnyeon-ro, Yeonggwang-eup, Yeonggwang-gun, Jeollanam-do 57049, South Korea., Cho H; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea., Hong SH; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea., Kim HJ; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea. Electronic address: rex7878@korea.kr., Lee SH; Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea. Electronic address: shlee96@korea.kr.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2022 Aug 02; Vol. 1676, pp. 463257. Date of Electronic Publication: 2022 Jun 23.
DOI: 10.1016/j.chroma.2022.463257
Abstrakt: Modified QuEChERS and triple quadrupole mass spectrometry (LC and GC-MS/MS) technology were used to sequentially analyze pesticides, veterinary drugs, and mycotoxins in feed. In order to analyze the harmful substances that may remain or occur in the feed, we performed optimization experiments for sample preparation and LC-MS/MS and GC-MS/MS conditions. Optimized sample preparation involves extracting 5 g of sample with 15 mL of 0.25 M EDTA and 10 mL of acetonitrile. And some extracts were diluted 10-fold with 100 mM ammonium formate aqueous solution and analyzed by LC-MS/MS, and some extracts were purified through 25 mg PSA and analyzed by GC-MS/MS by adding an analyte protectant. We confirmed the matrix effect of feed ingredients and compound feeds, and added a dilution process after extraction to increase on-site efficiency. Matrix-matched calibration was applied for quantification. Method validation was performed for 197 pesticides, 56 components for veterinary drugs, and 5 components for toxins. All the components showed good linearity (r2 ≥ 0.98) in the developed analytical method. For most compounds, the limit of quantitation was 0.05 mg/kg. The recovery rate experiment was repeated three times at three concentrations including LOQ in feed ingredient, compound feed for livestock, and compound feed for pets. The recovery rate was 70.09-119.76% and relative standard deviations were ≤ 18.91%. And the accuracy and precision were further verified through cross-validation between laboratories. The developed analytical method was used to monitor 414 domestically distributed and imported feeds.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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