A pan-genome and chromosome-length reference genome of narrow-leafed lupin (Lupinus angustifolius) reveals genomic diversity and insights into key industry and biological traits.

Autor: Garg G; CSIRO Agriculture and Food, Floreat, WA, 6014, Australia., Kamphuis LG; CSIRO Agriculture and Food, Floreat, WA, 6014, Australia.; UWA Institute of Agriculture, University of Western Australia, Crawley, WA, 6009, Australia.; Centre for Crop and Disease Management, Curtin University, Bentley, WA, 6102, Australia., Bayer PE; The School of Biological Sciences, University of Western Australia, Crawley, WA, 6009, Australia., Kaur P; School of Agriculture and Environment, University of Western Australia, Crawley, WA, 6009, Australia., Dudchenko O; Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, 77030, USA.; Center for Theoretical Biological Physics, Rice University, Houston, TX, 77005, USA., Taylor CM; UWA Institute of Agriculture, University of Western Australia, Crawley, WA, 6009, Australia.; School of Agriculture and Environment, University of Western Australia, Crawley, WA, 6009, Australia., Frick KM; CSIRO Agriculture and Food, Floreat, WA, 6014, Australia.; Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark., Foley RC; CSIRO Agriculture and Food, Floreat, WA, 6014, Australia., Gao LL; CSIRO Agriculture and Food, Floreat, WA, 6014, Australia., Aiden EL; School of Agriculture and Environment, University of Western Australia, Crawley, WA, 6009, Australia.; Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, 77030, USA.; Center for Theoretical Biological Physics, Rice University, Houston, TX, 77005, USA.; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech, Pudong, China.; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Edwards D; UWA Institute of Agriculture, University of Western Australia, Crawley, WA, 6009, Australia.; The School of Biological Sciences, University of Western Australia, Crawley, WA, 6009, Australia., Singh KB; CSIRO Agriculture and Food, Floreat, WA, 6014, Australia.; UWA Institute of Agriculture, University of Western Australia, Crawley, WA, 6009, Australia.; Centre for Crop and Disease Management, Curtin University, Bentley, WA, 6102, Australia.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2022 Sep; Vol. 111 (5), pp. 1252-1266. Date of Electronic Publication: 2022 Aug 11.
DOI: 10.1111/tpj.15885
Abstrakt: Narrow-leafed lupin (NLL; Lupinus angustifolius) is a key rotational crop for sustainable farming systems, whose grain is high in protein content. It is a gluten-free, non-genetically modified, alternative protein source to soybean (Glycine max) and as such has gained interest as a human food ingredient. Here, we present a chromosome-length reference genome for the species and a pan-genome assembly comprising 55 NLL lines, including Australian and European cultivars, breeding lines and wild accessions. We present the core and variable genes for the species and report on the absence of essential mycorrhizal associated genes. The genome and pan-genomes of NLL and its close relative white lupin (Lupinus albus) are compared. Furthermore, we provide additional evidence supporting LaRAP2-7 as the key alkaloid regulatory gene for NLL and demonstrate the NLL genome is underrepresented in classical NLR disease resistance genes compared to other sequenced legume species. The NLL genomic resources generated here coupled with previously generated RNA sequencing datasets provide new opportunities to fast-track lupin crop improvement.
(© 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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