Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies.

Autor: Kim SJ; Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USA., Yao Z; Donnelly Centre, University of Toronto, Ontario, Canada., Marsh MC; Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USA., Eckert DM; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA., Kay MS; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA., Lyakisheva A; Donnelly Centre, University of Toronto, Ontario, Canada., Pasic M; Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario, Canada.; Department of Laboratory Medicine, St. Joseph's Health Centre, Toronto, Ontario, Canada.; Centre for Global Health Research, Unity Health Toronto, University of Toronto, Toronto, Canada., Bansal A; Centre for Global Health Research, Unity Health Toronto, University of Toronto, Toronto, Canada., Birnboim C; Centre for Global Health Research, Unity Health Toronto, University of Toronto, Toronto, Canada., Jha P; Centre for Global Health Research, Unity Health Toronto, University of Toronto, Toronto, Canada., Galipeau Y; Department of Biochemistry, Microbiology & Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada., Langlois MA; Department of Biochemistry, Microbiology & Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada.; University of Ottawa Centre for Infection, Immunity and Inflammation (CI3), Ottawa, Canada., Delgado JC; ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.; Department of Pathology, University of Utah, Salt Lake City, UT, USA., Elgort MG; ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA., Campbell RA; Department of Pathology, University of Utah, Salt Lake City, UT, USA.; Department of Internal Medicine, Division of General Medicine, University of Utah, Salt Lake City, UT, USA.; Molecular Medicine Program, University of Utah, Salt Lake City, UT, USA., Middleton EA; Department of Internal Medicine, Division of General Medicine, University of Utah, Salt Lake City, UT, USA.; Molecular Medicine Program, University of Utah, Salt Lake City, UT, USA.; Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Utah, Salt Lake City, UT, USA., Stagljar I; Donnelly Centre, University of Toronto, Ontario, Canada. igor.stagljar@utoronto.ca.; Department of Biochemistry, University of Toronto, Ontario, Canada. igor.stagljar@utoronto.ca.; Department of Molecular Genetics, University of Toronto, Ontario, Canada. igor.stagljar@utoronto.ca.; Mediterranean Institute for Life Sciences, Meštrovićevo Šetalište 45, Split, Croatia. igor.stagljar@utoronto.ca.; School of Medicine, University of Split, Split, Croatia. igor.stagljar@utoronto.ca., Owen SC; Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USA. shawn.owen@hsc.utah.edu.; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, USA. shawn.owen@hsc.utah.edu.; Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT, USA. shawn.owen@hsc.utah.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Jul 01; Vol. 13 (1), pp. 3716. Date of Electronic Publication: 2022 Jul 01.
DOI: 10.1038/s41467-022-31300-9
Abstrakt: The COVID-19 pandemic triggered the development of numerous diagnostic tools to monitor infection and to determine immune response. Although assays to measure binding antibodies against SARS-CoV-2 are widely available, more specific tests measuring neutralization activities of antibodies are immediately needed to quantify the extent and duration of protection that results from infection or vaccination. We previously developed a 'Serological Assay based on a Tri-part split-NanoLuc® (SATiN)' to detect antibodies that bind to the spike (S) protein of SARS-CoV-2. Here, we expand on our previous work and describe a reconfigured version of the SATiN assay, called Neutralization SATiN (Neu-SATiN), which measures neutralization activity of antibodies directly from convalescent or vaccinated sera. The results obtained with our assay and other neutralization assays are comparable but with significantly shorter preparation and run time for Neu-SATiN. As the assay is modular, we further demonstrate that Neu-SATiN enables rapid assessment of the effectiveness of vaccines and level of protection against existing SARS-CoV-2 variants of concern and can therefore be readily adapted for emerging variants.
(© 2022. The Author(s).)
Databáze: MEDLINE
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