Urinary proteome of dogs with renal disease secondary to leishmaniosis.

Autor: González MA; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain. Electronic address: marioalbgs@unex.es., Barrera-Chacón R; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain., Peña FJ; Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, 10003 Cáceres, Spain., Fernández-Cotrina J; LeishmanCeres Laboratory (GLP Compliance Certified), Parasitology Unit, Veterinary Teaching Hospital, University of Extremadura, 10003 Cáceres, Spain., Robles NR; Nephrology Service, Badajoz University Hospital, University of Extremadura, 06080 Badajoz, Spain., Pérez-Merino EM; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain., Martín-Cano FE; Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, 10003 Cáceres, Spain., Duque FJ; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2022 Dec; Vol. 149, pp. 108-118. Date of Electronic Publication: 2022 Jun 25.
DOI: 10.1016/j.rvsc.2022.04.013
Abstrakt: Canine leishmaniosis is frequently associated with the development of renal disease. Its pathogenesis is complex and not fully understood. For this reason, this study aimed to describe the urinary proteome, and identify possible new biomarkers in dogs with kidney disease secondary to leishmaniosis. Urine samples were collected from 20 dogs, 5 from healthy dogs, and 15 from stages Leishvet III and IV. Urine samples were analyzed by UHPLC-MS/MS. The data are available via ProteomeXchange with identifier PXD029165. A total of 951 proteins were obtained. After bioinformatic analysis, 93 urinary proteins were altered in the study group. Enrichment analysis performed on these proteins showed an overrepresentation of the complement activation pathway, among others. Finally, 12 discriminant variables were found in dogs with renal disease secondary to leishmaniosis, highlighting C4a anaphylatoxin, apolipoprotein A-I, haptoglobin, leucine-rich alpha-2-glycoprotein 1, and beta-2-microglobulin. This study is the first to describe the urinary proteomics of dogs with renal disease caused by leishmaniosis, and it provides new possible biomarkers for the diagnosis and monitoring of this disease.
(Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: