Immuno-MALDI-MS for Accurate Quantitation of Targeted Peptides from Volume-Restricted Samples.
| Autor: | Sobsey CA; Segal Cancer Proteomics Centre, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, QC, Canada.; Division of Experimental Medicine, McGill University, Montreal, QC, Canada., Froehlich B; University of Victoria - Genome BC Proteomics Centre, Victoria, BC, Canada.; Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada., Batist G; Division of Experimental Medicine, McGill University, Montreal, QC, Canada.; Gerald Bronfman Department of Oncology, Jewish General Hospital, McGill University, Montreal, QC, Canada.; Exactis Innovation, Montreal, QC, Canada., Borchers CH; Segal Cancer Proteomics Centre, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, QC, Canada. Christoph.Borchers@mcgill.ca.; Gerald Bronfman Department of Oncology, Jewish General Hospital, McGill University, Montreal, QC, Canada. Christoph.Borchers@mcgill.ca. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2515, pp. 203-225. |
| DOI: | 10.1007/978-1-0716-2409-8_13 |
| Abstrakt: | The immuno-MALDI-MS method can be used to quantify low-abundance proteins from clinical samples that offer only a limited amount of material for analysis. An internal standard, in the form of a stable isotope-labeled peptide, is used to ensure reproducible and absolute quantitation. The protocol described here was optimized for the quantitation of AKT1 and AKT2, but we offer instructions on how to adapt the method to target other proteins. The described workflow is compatible with automation via a liquid handling robot for high-throughput applications. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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