How abiotic stress-induced socialization leads to the formation of massive aggregates in Chlamydomonas.

Autor:	de Carpentier F; Institut de Biologie Physico-Chimique, UMR 8226, CNRS, Sorbonne Université, 75005 Paris, France.; Institut de Biologie Paris-Seine, UMR 7238, CNRS, Sorbonne Université, 75005 Paris, France.; Université Paris-Saclay, 91190 Saint-Aubin, France., Maes A; Institut de Biologie Physico-Chimique, UMR 8226, CNRS, Sorbonne Université, 75005 Paris, France., Marchand CH; Institut de Biologie Physico-Chimique, UMR 8226, CNRS, Sorbonne Université, 75005 Paris, France.; Institut de Biologie Paris-Seine, UMR 7238, CNRS, Sorbonne Université, 75005 Paris, France., Chung C; Institut de Biologie Physico-Chimique, UMR 8226, CNRS, Sorbonne Université, 75005 Paris, France., Durand C; Institut de Biologie Physico-Chimique, UMR 8226, CNRS, Sorbonne Université, 75005 Paris, France., Crozet P; Institut de Biologie Physico-Chimique, UMR 8226, CNRS, Sorbonne Université, 75005 Paris, France.; Institut de Biologie Paris-Seine, UMR 7238, CNRS, Sorbonne Université, 75005 Paris, France.; Polytech-Sorbonne, Sorbonne Université, 75005 Paris, France., Lemaire SD; Institut de Biologie Physico-Chimique, UMR 8226, CNRS, Sorbonne Université, 75005 Paris, France.; Institut de Biologie Paris-Seine, UMR 7238, CNRS, Sorbonne Université, 75005 Paris, France., Danon A; Institut de Biologie Physico-Chimique, UMR 8226, CNRS, Sorbonne Université, 75005 Paris, France.; Institut de Biologie Paris-Seine, UMR 7238, CNRS, Sorbonne Université, 75005 Paris, France.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2022 Oct 27; Vol. 190 (3), pp. 1927-1940.
DOI:	10.1093/plphys/kiac321
Abstrakt:	Multicellular organisms implement a set of reactions involving signaling and cooperation between different types of cells. Unicellular organisms, on the other hand, activate defense systems that involve collective behaviors between individual organisms. In the unicellular model alga Chlamydomonas (Chlamydomonas reinhardtii), the existence and the function of collective behaviors mechanisms in response to stress remain mostly at the level of the formation of small structures called palmelloids. Here, we report the characterization of a mechanism of abiotic stress response that Chlamydomonas can trigger to form massive multicellular structures. We showed that these aggregates constitute an effective bulwark within which the cells are efficiently protected from the toxic environment. We generated a family of mutants that aggregate spontaneously, the socializer (saz) mutants, of which saz1 is described here in detail. We took advantage of the saz mutants to implement a large-scale multiomics approach that allowed us to show that aggregation is not the result of passive agglutination, but rather genetic reprogramming and substantial modification of the secretome. The reverse genetic analysis we conducted allowed us to identify positive and negative regulators of aggregation and to make hypotheses on how this process is controlled in Chlamydomonas. (© American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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