Vaginal microbiota associated with oncogenic HPV in a cohort of HPV-vaccinated women living with HIV.

Autor: McClymont E; Department of Obstetrics and Gynecology, 198113University of British Columbia, Vancouver, BC, Canada.; 469220Canadian HIV Trials Network, Vancouver, BC, Canada., Albert AY; 574481Women's Health Research Institute, Vancouver, BC, Canada., Wang C; Faculty of Medicine, 12358University of British Columbia, Vancouver, BC, Canada., Dos Santos SJ; Department of Veterinary Microbiology, 70399University of Saskatchewan, Saskatoon, SK, Canada., Coutlée F; Département de Microbiologie Médicale et Infectiologie, 5622l'Université de Montréal, Montréal, QC, Canada., Lee M; Department of Obstetrics and Gynecology, 198113University of British Columbia, Vancouver, BC, Canada., Walmsley S; Toronto General Hospital Research Institute, University of Toronto, 7989University Health Network, Toronto, ON, Canada.; Dalla Lana School of Public Health, 274071University of Toronto, Toronto, ON, Canada., Lipsky N; 574481Women's Health Research Institute, Vancouver, BC, Canada., Loutfy M; Women's College Research Institute, University of Toronto, Toronto, ON, Canada., Trottier S; Infectious Diseases Research Centre, 4440Université Laval, Québec City, QC, Canada., Smaill F; Department of Pathology and Molecular Medicine, 3710McMaster University, Hamilton, ON, Canada., Klein MB; 54473McGill University Health Centre, Montreal, QC, Canada., Yudin MH; Women's College Research Institute, University of Toronto, Toronto, ON, Canada.; Department of Obstetrics and Gynecology, University of Toronto, 574538St. Michael's Hospital, Toronto, ON, Canada., Harris M; Faculty of Medicine, 12358University of British Columbia, Vancouver, BC, Canada.; 198129British Columbia Centre for Excellence in HIV/AIDS, Vancouver, BC, Canada., Wobeser W; Departments of Public Health and Molecular & Biomedical Sciences, 4257Queen's University, Kingston, ON, Canada., Hill JE; Department of Veterinary Microbiology, 70399University of Saskatchewan, Saskatoon, SK, Canada., Money DM; Department of Obstetrics and Gynecology, 198113University of British Columbia, Vancouver, BC, Canada.; 574481Women's Health Research Institute, Vancouver, BC, Canada.
Jazyk: angličtina
Zdroj: International journal of STD & AIDS [Int J STD AIDS] 2022 Aug; Vol. 33 (9), pp. 847-855. Date of Electronic Publication: 2022 Jul 01.
DOI: 10.1177/09564624221109686
Abstrakt: Background: Women living with HIV (WLWH) experience higher rates of human papillomavirus (HPV) infection and cervical cancer than women without HIV. Changes in the vaginal microbiome have been implicated in HPV-related disease processes such as persistence of high-risk HPV infection but this has not been well defined in a population living with HIV.
Methods: Four hundred and 20 girls and WLWH, age ≥9, across 14 clinical sites in Canada were enrolled to receive three doses of quadrivalent HPV vaccine for assessment of vaccine immunogenicity. Blood, cervical cytology, and cervico-vaginal swabs were collected. Cervico-vaginal samples were tested for HPV DNA and underwent microbiota sequencing.
Results: Principal component analysis (PCA) and hierarchical clustering generated community state types (CSTs). Relationships between taxa and CSTs with HPV infection were examined using mixed-effects logistic regressions, Poisson regressions, or generalized linear mixed-effects models, as appropriate. Three hundred and fifty-six cervico-vaginal microbiota samples from 172 women were sequenced. Human papillomavirus DNA was detected in 211 (59%) samples; 110 (31%) contained oncogenic HPV. Sixty-five samples (18%) were taken concurrently with incident oncogenic HPV infection and 56 (16%) were collected from women with concurrent persistent oncogenic HPV infection.
Conclusions: No significant associations between taxa, CST, or microbial diversity and HPV-related outcomes were found. However, we observed weak associations between a dysbiotic microbiome and specific species, including Gardnerella , Porphyromonas , and Prevotella species, with incident HPV infection.
Databáze: MEDLINE