Visualizing retinal cells with adaptive optics imaging modalities using a translational imaging framework.

Autor:	Giannini JP; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Lu R; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Bower AJ; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Fariss R; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Tam J; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2022 Apr 25; Vol. 13 (5), pp. 3042-3055. Date of Electronic Publication: 2022 Apr 25 (Print Publication: 2022).
DOI:	10.1364/BOE.454560
Abstrakt:	Adaptive optics reflectance-based retinal imaging has proved a valuable tool for the noninvasive visualization of cells in the living human retina. Many subcellular features that remain at or below the resolution limit of current in vivo techniques may be more easily visualized with the same modalities in an ex vivo setting. While most microscopy techniques provide significantly higher resolution, enabling the visualization of fine cellular detail in ex vivo retinal samples, they do not replicate the reflectance-based imaging modalities of in vivo retinal imaging. Here, we introduce a strategy for imaging ex vivo samples using the same imaging modalities as those used for in vivo retinal imaging, but with increased resolution. We also demonstrate the ability of this approach to perform protein-specific fluorescence imaging and reflectance imaging simultaneously, enabling the visualization of nearly transparent layers of the retina and the classification of cone photoreceptor types. Competing Interests: The authors declare that there are no conflicts of interest related to this article. (© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.)
Databáze:	MEDLINE
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