Preovulatory follicular fluid and serum metabolome profiles in lactating beef cows with thin, moderate, and obese body condition.

Autor:	Horn EJ; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Read CC; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Edwards JL; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Schrick FN; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Rhinehart JD; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Payton RR; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Campagna SR; Department of Chemistry, University of Tennessee, Knoxville, TN 37996, USA., Klabnik JL; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Clark HM; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Myer PR; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., McLean KJ; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Moorey SE; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA.
Jazyk:	angličtina
Zdroj:	Journal of animal science [J Anim Sci] 2022 Jul 01; Vol. 100 (7).
DOI:	10.1093/jas/skac152
Abstrakt:	Extremes in body condition reduce fertility and overall productivity in beef cattle herds, due in part to altered systemic metabolic conditions that influence the intrafollicular and uterine environment. Follicular fluid and serum metabolome profiles are influenced by body composition in women and dairy cattle; however, such information is lacking in beef cattle. We hypothesized that body condition score (BCS)-related alterations in the metabolome of preovulatory follicular fluid and serum may influence oocyte maturation while impacting the oviductal or uterine environment. Therefore, we performed a study with the objective to determine the relationship between BCS and the metabolome of follicular fluid and serum in lactating beef cattle. We synchronized the development of a preovulatory follicle in 130 cows of varying BCS. We collected blood and performed transvaginal follicle aspirations to collect follicular fluid from the preovulatory follicle ~18 h after gonadotropin-releasing hormone administration to stimulate the preovulatory gonadotropin surge. We then selected follicular fluid and serum samples from cows with BCS 4 (Thin; n = 14), BCS 6 (Moderate; n = 18), or BCS >8 (Obese; n = 14) for ultra-high performance liquid chromatography-high resolution mass spectrometry. We identified differences in the follicular fluid or serum of thin, moderate, and obese animals based on multiple linear regression. MetaboAnalyst 5.0 was used for enrichment analysis of significant metabolites. We identified 38 metabolites in follicular fluid and 49 metabolites in serum. There were no significant differences in follicular fluid metabolite content among BCS classifications. There were 5, 22, and 1 serum metabolites differentially abundant between thin-obese, moderate-thin, and moderate-obese classifications, respectively (false discovery rate [FDR] < 0.10). These metabolites were enriched in multiple processes including "arginine biosynthesis," "arginine/proline metabolism," and "D-glutamine/D-glutamate metabolism" (FDR < 0.04). Pathways enriched with serum metabolites associated with BCS indicate potentially increased reactive oxygen species (ROS) in serum of thin cows. ROS crossing the blood follicular barrier may negatively impact the oocyte during oocyte maturation and contribute to the reduced pregnancy rates observed in thin beef cows. (© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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