Assessing the Redox Status of Mitochondria Through the NADH/FAD 2 + Ratio in Intact Cells.

Autor: Chi H; Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK., Bhosale G; Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK., Duchen MR; Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK. m.duchen@ucl.ac.uk.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2497, pp. 313-318.
DOI: 10.1007/978-1-0716-2309-1_21
Abstrakt: This section aims to describe the measurement of NADH and FAD 2 + levels in intact cells using fluorescence microscopy. Both NADH and FADH 2 are major electron donors for the electron transport chain through shifting of their redox status. Furthermore, within their redox couples, only NADH and FAD 2+ are fluorescent. Therefore, calibration of the NADH and FAD 2+ fluorescence signal is a crucial factor in accurately assessing mitochondrial function and redox status.
(© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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