Cerium Oxide Nanoparticles Biosynthesized Using Fresh Green Walnut Shell in Microwave Environment and their Anticancer Effect on Breast Cancer Cells.

Autor: Sulak M; Department of Mathematics and Science Education, Faculty of Education, University of Pamukkale, 20070, Denizli, Turkey., Turgut GC; Department of Organic Farming Business Management, Faculty of Applied Sciences, University of Pamukkale, 20600, Civril, Denizli, Turkey., Sen A; Department of Biology, Faculty of Arts & Sciences, University of Pamukkale, 20070, Denizli, Turkey.; Department of Molecular Biology and Genetics, Faculty of Life and Natural Sciences, University of Abdullah Gul, 38080, Kayseri, Turkey.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2022 Aug; Vol. 19 (8), pp. e202200131. Date of Electronic Publication: 2022 Jul 12.
DOI: 10.1002/cbdv.202200131
Abstrakt: In this study, cerium oxide nanoparticles (CONPs) were synthesized using fresh green walnut shell extract in microwave environment. The morphology and structure of the CONPs were determined using ultraviolet-visible (UV/VIS), attenuated total reflection-Fourier transform infrared (ATR-FT-IR), X-ray diffraction (XRD), energy-dispersive X-ray (EDX) spectroscopy, and scanning electron microscopy (SEM). Crystal purple staining, Annexin V-FITC detection, RT-PCR, P53, and NF-κB luciferase reporter assays were performed to evaluate the mechanism of action of CONPs in breast cancer cell lines (MCF7). The biosynthesized CONPs showed cytotoxic effects and induced apoptosis in MCF7 cells. Furthermore, CONPs induced P53 expression and suppressed NF-κB gene expression, both of which were confirmed using reporter assays. Based on the present results, it was concluded that CONPs can induce apoptosis by acting on P53 at the transcriptional level and may cause cell death by suppressing NF-κB-mediated transcription.
(© 2022 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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