Peripubertal requirement of Tsg101 in maintaining the integrity of membranous structures in mouse oocytes.

Autor: Shin H; Department of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.; Korean Institute of Oriental Medicine, Daejeon, Republic of Korea., Park D; Department of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.; Maria S Fertility Hospital, Seoul, Republic of Korea., Kim J; Department of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.; Maria Plus Fertility Hospital, Seoul, Republic of Korea., Nam MY; Department of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.; Maria Fertility Hospital, Seoul, Republic of Korea., Kwon S; Department of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea., Um DE; Department of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.; Maria Fertility Hospital, Seoul, Republic of Korea., Oh JE; Department of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea., Youn E; Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea., Shim YH; Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea., Wagner KU; Wayne State University School of Medicine and Tumor Biology Program, Barbara Ann Karmanos Cancer Institute, Detroit, Michigan, USA., Jun JH; Department of Biomedical Laboratory Science, Eulji University, Seongnam, Gyeonggi-do, Republic of Korea., Kim HR; Department of Biomedical Science, CHA University, Seongnam, Gyeonggi-do, Republic of Korea., Song H; Department of Biomedical Science, CHA University, Seongnam, Gyeonggi-do, Republic of Korea., Lim HJ; Department of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
Jazyk: angličtina
Zdroj: Cell proliferation [Cell Prolif] 2022 Oct; Vol. 55 (10), pp. e13288. Date of Electronic Publication: 2022 Jun 29.
DOI: 10.1111/cpr.13288
Abstrakt: Objective: As a component of Endosomal Sorting Complex Required for Transport (ESCRT) complex I, the tumor susceptibility gene 101 (Tsg101) carries out multiple functions. In this work, we report that oocyte-specific deletion of tumor susceptibility gene 101 (Tsg101) leads to age-dependent oocyte demise in mice.
Materials and Method: Tsg101 floxed mice (Tsg101 f/f ) were bred with Zp3 cre transgenic mice to examine oocyte-specific roles of Tsg101. Multiple cellular and molecular biological approaches were taken to examine what leads to oocyte demise in the absence of Tsg101.
Results: The death of oocytes from Zp3 cre /Tsg101 f/f (Tsg101 d/d thereafter) mice showed a strong correlation with sexual maturation, as gonadotropin-releasing hormone antagonist injections improved the survival rate of oocytes from 5-week-old Tsg101 d/d mice. Maturation of oocytes from prepubertal Tsg101 d/d mice proceeded normally, but was largely abnormal in oocytes from peripubertal Tsg101 d/d mice, showing shrinkage or rupture. Endolysosomal structures in oocytes from peripubertal Tsg101 d/d mice showed abnormalities, with aberrant patterns of early and late endosomal markers and a high accumulation of lysosomes. Dying oocytes showed plasma membrane blebs and leakage. Blockage of endocytosis in oocytes at 4°C prevented cytoplasmic shrinkage of oocytes from Tsg101 d/d mice until 9 h. The depletion of tsg-101 in Caenorhabditis elegans increased the permeability of oocytes and embryos, suggesting a conserved role of Tsg101 in maintaining membrane integrity.
Conclusions: Collectively, Tsg101 plays a dual role in maintaining the integrity of membranous structures, which is influenced by age in mouse oocytes.
(© 2022 The Authors. Cell Proliferation published by European Cell Proliferation Society and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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