Contribution of tick-borne diseases to mortality in juvenile free-living cervids.

Autor:	Dwużnik-Szarek D; Department of Eco-Epidemiology of Parasitic Diseases, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Poland., Kowalec M; Department of Eco-Epidemiology of Parasitic Diseases, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Poland., Alsarraf M; Department of Eco-Epidemiology of Parasitic Diseases, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Poland., Bajer A; Department of Eco-Epidemiology of Parasitic Diseases, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Poland.
Jazyk:	angličtina
Zdroj:	Annals of agricultural and environmental medicine : AAEM [Ann Agric Environ Med] 2022 Jun 24; Vol. 29 (2), pp. 215-219. Date of Electronic Publication: 2021 Oct 21.
DOI:	10.26444/aaem/142513
Abstrakt:	Introduction and Objective: Reports on tick-borne infections in free-living juvenile animals and their impact on survival of cervids in nature are lacking. The aim of the study was to detect and identify the Babesia and Anaplasma phagocytophilum species/ecotypes that may have contributed to the death of juvenile animals from a wildlife rescue centre in spring 2020. Material and Methods: PCR amplification and sequencing of two genetic markers (18S rDNA and cox1 for Babesia , 16S rDNA and groEL for A. phagocytophilum ) were used for screening eleven samples derived from juvenile animals which died in a rescue centre (seven roe deer Capreolus capreolus , one elk Alces alces , one red squirrel Sciurus vulgaris , one European beaver Castor fiber , one red fox Vulpes vulpes ). Phylogenetic analysis of full-length 18S rDNA sequence was performed to enable differentiation between two closely-related species infecting wild ungulates, Babesia capreoli and Babesia divergens (zoonotic). Results: The occurrence of the typical SNPs of B. capreoli at two discriminating positions in the 18S rRNA gene allowed identification of B. capreoli infection in a roe deer calf. In two calves, Anaplasma phagocytophilum ecotype 2 was identified, including the same calf co-infection. No Babesia DNA was amplified in an elk calf treated for babesiosis. Splenomegaly was recorded in roe deer calves with A. phagocytophilum and A. phagocytophilum + B. capreoli infection. Conclusions: The study revealed that tick-borne infections are common among juvenile, free-living cervids and may contribute to their mortality.
Databáze:	MEDLINE
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