Intracellular connections between basal bodies promote the coordinated behavior of motile cilia.

Autor:	Soh AWJ; Department of Cell and Developmental Biology, Anschutz Medical Campus, University of Colorado, Aurora, CO 80045., Woodhams LG; Department of Mechanical Engineering and Material Science, Washington University in St. Louis, St. Louis, MO 63130., Junker AD; Department of Cell and Developmental Biology, Anschutz Medical Campus, University of Colorado, Aurora, CO 80045., Enloe CM; Department of Chemical Engineering, College of Engineering and Applied Science, University of Wyoming, Laramie, WY 82071., Noren BE; Department of Chemical Engineering, College of Engineering and Applied Science, University of Wyoming, Laramie, WY 82071., Harned A; Center for Molecular Microscopy and Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, and., Westlake CJ; Laboratory of Cell and Developmental Signaling, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702., Narayan K; Center for Molecular Microscopy and Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, and., Oakey JS; Department of Chemical Engineering, College of Engineering and Applied Science, University of Wyoming, Laramie, WY 82071., Bayly PV; Department of Mechanical Engineering and Material Science, Washington University in St. Louis, St. Louis, MO 63130., Pearson CG; Department of Cell and Developmental Biology, Anschutz Medical Campus, University of Colorado, Aurora, CO 80045.
Jazyk:	angličtina
Zdroj:	Molecular biology of the cell [Mol Biol Cell] 2022 Sep 15; Vol. 33 (11), pp. br18. Date of Electronic Publication: 2022 Jun 29.
DOI:	10.1091/mbc.E22-05-0150
Abstrakt:	Hydrodynamic flow produced by multiciliated cells is critical for fluid circulation and cell motility. Hundreds of cilia beat with metachronal synchrony for fluid flow. Cilia-driven fluid flow produces extracellular hydrodynamic forces that cause neighboring cilia to beat in a synchronized manner. However, hydrodynamic coupling between neighboring cilia is not the sole mechanism that drives cilia synchrony. Cilia are nucleated by basal bodies (BBs) that link to each other and to the cell's cortex via BB-associated appendages. The intracellular BB and cortical network is hypothesized to synchronize ciliary beating by transmitting cilia coordination cues. The extent of intracellular ciliary connections and the nature of these stimuli remain unclear. Moreover, how BB connections influence the dynamics of individual cilia has not been established. We show by focused ion beam scanning electron microscopy imaging that cilia are coupled both longitudinally and laterally in the ciliate Tetrahymena thermophila by the underlying BB and cortical cytoskeletal network. To visualize the behavior of individual cilia in live, immobilized Tetrahymena cells, we developed D elivered I ron P article U biety L ive L ight (DIPULL) microscopy. Quantitative and computer analyses of ciliary dynamics reveal that BB connections control ciliary waveform and coordinate ciliary beating. Loss of BB connections reduces cilia-dependent fluid flow forces.
Databáze:	MEDLINE
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