Could Argyrophilic Nucleolar Organizer Regions count mirror DNA Ploidy in Malignant Salivary Gland Tumors?

Autor:	Hussien MT; Department of Oncologic Pathology, South Egypt Cancer Institute Assiut University, Assiut, Egypt., Mohamed MA; Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Sphinx University, Assiut, Egypt., Mansor SG; Department of Clinical Pathololgy, South Egypt Cancer Institute Assiut University, Assiut, Egypt., Temerik DF; Department of Clinical Pathololgy, South Egypt Cancer Institute Assiut University, Assiut, Egypt., Elgayar SF; Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Al Minia, Egypt., Abd El-Aziz EAE; Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Al Minia, Egypt.
Jazyk:	angličtina
Zdroj:	Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2022 Jun 01; Vol. 23 (6), pp. 1983-1992. Date of Electronic Publication: 2022 Jun 01.
DOI:	10.31557/APJCP.2022.23.6.1983
Abstrakt:	Objective: Nucleolar organizer regions (NORs) are DNA coils that transcribe to ribosomal RNA. The NOR-associated protein, termed argyrophilic NOR (AgNOR), was visible within the nucleus by staining with silver nitrate examination via the light microscope. AgNOR counting is a proliferation marker and may help in the diagnosis and prognosis of various neoplastic lesions. Aneuploidy (abnormal DNA content) can predict the progression, survival and prognosis of the tumors. The aim of this study was to evaluate the role of AgNORs, DNA ploidy status, and total S-phase fraction (TSPF) as prognostic parameters in malignant salivary gland tumors (MSGTs). Methods: The current study is a retrospective study on a cohort of MSGTs (N=47), to assess AgNORs using Silver Nitrate stain, DNA index (DI), and TSPF using flow cytometry (FCM). Data including tumor size and site, lymphovascular invasion (LVI), lymph node metastasis (LNM) were collected. Results: The AgNORs count was statistically significant with MSGT type. DI was found to have a significant association with tumor site, tumor size and MSGT type. In addition, TSPF was found to be significantly associated with LVI. A moderate positive correlation was noted between AgNORs count and TSPF. LNM, tumor site, high AgNORs and low DI were all associated with short disease-free survival (DFS) and poor overall survival (OS). Conclusion: The present study revealed that high AgNORs count, DNA aneuploidy and TSPF had a poor influence on MSGTs prognosis.
Databáze:	MEDLINE
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