Proteomic analysis of chemically transformed NIH-3T3 cells reveals novel mechanisms of action of amaranth lunasin-like peptide.

Autor: Mazorra-Carrillo JL; IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José No. 2055, Lomas 4ª Sección, 78216 San Luis Potosí, S.L.P, México., De León-Rodríguez A; IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José No. 2055, Lomas 4ª Sección, 78216 San Luis Potosí, S.L.P, México., Huerta-Ocampo JA; IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José No. 2055, Lomas 4ª Sección, 78216 San Luis Potosí, S.L.P, México., Velarde-Salcedo AJ; IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José No. 2055, Lomas 4ª Sección, 78216 San Luis Potosí, S.L.P, México., González de Mejía E; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, IL, USA. Electronic address: edemejia@illinois.edu., Barba de la Rosa AP; IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José No. 2055, Lomas 4ª Sección, 78216 San Luis Potosí, S.L.P, México. Electronic address: apbarba@ipicyt.edu.mx.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2022 Jul; Vol. 157, pp. 111374. Date of Electronic Publication: 2022 May 14.
DOI: 10.1016/j.foodres.2022.111374
Abstrakt: Food-derived biopeptides can interact with genes and proteins to preserve health and prevent the development of diseases. Lunasin is a soybean cancer-preventive peptide that has been well characterized; however, few studies have been carried out to characterize the function of amaranth lunasin-like peptide (AhLun). The aim of this work was to analyze the proteomic profile changes in NIH-3T3 cells when they are chemically transformed with the carcinogen 3-methylcholanthrene (3MC) in the absence or presence of AhLun. The addition of AhLun into the culture medium did not affect the cell morphology; however, as a chemopreventive agent, it significantly reduced anisokaryosis formation when cells were treated with 3MC. Changes in protein accumulation in NIH-3T3 cells were evaluated by gel-based proteomics analysis. Differentially accumulated protein spots that exhibited at least a twofold change in spot intensity (p < 0.05), when compared with control cells, were analyzed by LC-MS/MS. Successfully identified proteins were grouped into six main categories according to their localization and function (nuclear, ribosomal, mitochondrial, metabolism, cytoskeletal, and miscellaneous). The gel-based proteomic approach for the evaluation of the chemopreventive potential of AhLun reveals novel pathways of action and provides new clues about the possible mechanisms of action of this bioactive peptide present in amaranth seeds.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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