Molecular mechanics underlying flat-to-round membrane budding in live secretory cells.

Autor: Shin W; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA., Zucker B; Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 69978, Ramat Aviv, Israel., Kundu N; Structural Cell Biology Section, Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA., Lee SH; Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, Republic of Korea., Shi B; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA., Chan CY; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA., Guo X; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA., Harrison JT; Structural Cell Biology Section, Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA., Turechek JM; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA., Hinshaw JE; Structural Cell Biology Section, Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA. jennyh@niddk.nih.gov., Kozlov MM; Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 69978, Ramat Aviv, Israel. michk@tauex.tau.ac.il., Wu LG; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA. wul@ninds.nih.gov.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Jun 27; Vol. 13 (1), pp. 3697. Date of Electronic Publication: 2022 Jun 27.
DOI: 10.1038/s41467-022-31286-4
Abstrakt: Membrane budding entails forces to transform flat membrane into vesicles essential for cell survival. Accumulated studies have identified coat-proteins (e.g., clathrin) as potential budding factors. However, forces mediating many non-coated membrane buddings remain unclear. By visualizing proteins in mediating endocytic budding in live neuroendocrine cells, performing in vitro protein reconstitution and physical modeling, we discovered how non-coated-membrane budding is mediated: actin filaments and dynamin generate a pulling force transforming flat membrane into Λ-shape; subsequently, dynamin helices surround and constrict Λ-profile's base, transforming Λ- to Ω-profile, and then constrict Ω-profile's pore, converting Ω-profiles to vesicles. These mechanisms control budding speed, vesicle size and number, generating diverse endocytic modes differing in these parameters. Their impact is widespread beyond secretory cells, as the unexpectedly powerful functions of dynamin and actin, previously thought to mediate fission and overcome tension, respectively, may contribute to many dynamin/actin-dependent non-coated-membrane buddings, coated-membrane buddings, and other membrane remodeling processes.
(© 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
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