Chemotaxonomy and Antibacterial Activity of the Extracts and Chemical Constituents of Psychotria succulenta Hiern. (Rubiaceae).

Autor: Ngnokam Jouogo DC; Research Unit of Applied and Environmental Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Tamokou JD; Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Teponno RB; Research Unit of Applied and Environmental Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Matsuete-Takongmo G; Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Voutquenne-Nazabadioko L; Groupe Isolement et Structure, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, Bat. 18, B.P. 1039, 51687 Reims Cedex 2, France., Tapondjou LA; Research Unit of Applied and Environmental Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Ngnokam D; Research Unit of Applied and Environmental Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2022 Jun 15; Vol. 2022, pp. 7856305. Date of Electronic Publication: 2022 Jun 15 (Print Publication: 2022).
DOI: 10.1155/2022/7856305
Abstrakt: The use of natural products for medicinal purposes is becoming more and more common nowadays, as evidenced by the presence in plants of secondary metabolites with different potentials such as antioxidant and antibacterial properties. We evaluated in this work the antimicrobial activities of the extracts and some isolated compounds from the seeds of Psychotria succulenta Hiern. (Rubiaceae), a Cameroonian medicinal plant traditionally used to cure microbial infections. The ethanol extract was prepared by maceration and extracted with ethyl acetate and n -butanol. The EtOAc ( m = 168 g) and n -BuOH ( m = 20 g) extracts were further fractionated by silica gel column chromatography to isolation of compounds. Their structures were elucidated by spectroscopic analysis and by comparison with published data. The antibacterial activity of extracts and compounds was assessed by evaluating the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against pathogenic bacteria. Thirteen compounds including four alkaloids (veprisine ( 1 ), naucleofficine III ( 2 ), vepridimerine B ( 3 ), and vepridimerine C ( 4 )), three triterpenes (barbinervic acid ( 5 ), 3- O - α -L-rhamnopyranosyl quinovic acid ( 6 ), and oleanolic acid ( 7 )), one steroid ( β -sitosterol-3- O - β -D-glucopyranoside ( 8 )), four phenolic compounds (scopoletin ( 9 ), gallic acid ( 10 ), quercetin-3- O - β -D-glucopyranoside ( 11 ), and kaempferol 3- O - α -L-rhamnopyranoside-7- O - α -L-rhamnopyranoside ( 12 )), and one iridoid (borreriagenin ( 13 )) were isolated from the EtOAc and n -BuOH extracts. These compounds were identified by 1D and 2D NMR combined analysis as well as by melting point comparison. The EtOH, EtOAc, and n -BuOH extracts exhibited significant antibacterial activities (MIC = 32-128  μ g/mL; MBC = 64-256  μ g/mL) against Staphylococcus aureus (Gram-positive bacterium), Pseudomonas aeruginosa , Escherichia coli , and Klebsiella pneumonia (Gram-negative bacteria). Among the isolated compounds, scopoletin ( 9 ) showed a moderate activity against Klebsiella pneumoniae with MIC and MBC values of 16  μ g/mL and 32  μ g/mL, respectively. It appears that, chemotaxonomically, some of the isolated compounds have already been obtained from the genus Psychotria but to the best of our knowledge, this is the first report on the phytochemical investigation of P. succulenta . Although many other studies need to be achieved, our results support the use of P. succulenta in traditional medicine to cure infectious diseases particularly those caused by the tested bacteria.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2022 Darille Claudia Ngnokam Jouogo et al.)
Databáze: MEDLINE
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