Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa.

Autor:	Pokam BDT; Department of Medical Laboratory Science, University of Buea, Buea, Cameroon.; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Yeboah-Manu D; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Ofori S; Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Guemdjom PW; Department of Public Health, University of Buea, Buea, Cameroon., Teyim PM; Douala Tuberculosis Reference Laboratory, Littoral Region, Cameroon., Lawson L; Zankli Medical Centre, Utako, Abuja, Nigeria., Amiteye D; Department of Biomedical Engineering, All Nations University College, Koforidua, Ghana., Yhiler NY; Department of Medical Laboratory Science, University of Buea, Buea, Cameroon.; Department of Allied Health, Biaka University Institute, Buea, Cameroon., Djuikoue IC; Microbiology Department, Faculty of Health Sciences, Université des Montagnes, Bangangte, Cameroon.; Foundation Prevention and Control, Cameroon., Asuquo AE; Department of Medical Laboratory Science, College of Medicine, University of Calabar, Calabar, Nigeria.
Jazyk:	angličtina
Zdroj:	IJID Regions (Online) [IJID Reg] 2022 May 14; Vol. 3, pp. 287-292. Date of Electronic Publication: 2022 May 14 (Print Publication: 2022).
DOI:	10.1016/j.ijregi.2022.05.003
Abstrakt:	Objective: Differentiation between non-tuberculous mycobacteria ( NTM) and Mycobacterium tuberculosis complex (MTBC) is crucial for case management with the appropriate antimycobacterials. This study was undertaken in three West and Central African countries to understand NTM associated with pulmonary tuberculosis in the sub-region. Methods: A collection of 503 isolates (158 from Cameroon, 202 from Nigeria and 143 from Ghana) obtained from solid and liquid cultures were analysed. The isolates were tested for drug susceptibility, and MTBC were confirmed using IS 6110 . All IS 6110 -negative isolates were identified by 65-kilodalton heat shock protein ( hsp65 ) gene amplification, DNA sequencing and BLAST analysis. Results: Overall, the prevalence of NTM was 16/503 (3.2%), distributed as 2/202 (1%) in Nigeria, 2/158 (1.3%) in Cameroon and 12/143 (8.4%) in Ghana. The main NTM isolates included 5/16 (31.3%) M. fortuitum , 2/16 (12.5%) M. intracellulare and 2/16 (12.5%) M. engbaekii. Eight (57.1%) of the 14 previously treated patients harboured NTM (odds ratio 0.21, 95% confidence interval 0.06-0.77; P =0.021). Three multi-drug-resistant strains were identified: M. engbaekii, M. fortuitum and M. intracellulare. Conclusion: NTM were mainly found among individuals with unsuccessful treatment. This highlights the need for mycobacterial species differentiation using rapid molecular tools for appropriate case management, as most are resistant to routine first-line antimycobacterials. Competing Interests: None declared. (© 2022 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.)
Databáze:	MEDLINE
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