Chemical synthesis of Torenia plant pollen tube attractant proteins by KAHA ligation.

Autor:	Kumarswamyreddy N; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8601 Japan bode@itbm.nagoya-u.ac.jp oishi@itbm.nagoya-u.ac.jp.; Department of Chemistry, Indian Institute of Technology Tirupati Tirupati Andhra Pradesh 517506 India., Reddy DN; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8601 Japan bode@itbm.nagoya-u.ac.jp oishi@itbm.nagoya-u.ac.jp.; Medicinal & Process Chemistry Division, CSIR-Central Drug Research Institute Lucknow 226031 India., Robkis DM; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8601 Japan bode@itbm.nagoya-u.ac.jp oishi@itbm.nagoya-u.ac.jp., Kamiya N; Division of Biological Science, Graduate School of Science, Nagoya University Nagoya 464-0602 Japan., Tsukamoto R; Division of Biological Science, Graduate School of Science, Nagoya University Nagoya 464-0602 Japan., Kanaoka MM; Division of Biological Science, Graduate School of Science, Nagoya University Nagoya 464-0602 Japan., Higashiyama T; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8601 Japan bode@itbm.nagoya-u.ac.jp oishi@itbm.nagoya-u.ac.jp.; Division of Biological Science, Graduate School of Science, Nagoya University Nagoya 464-0602 Japan.; Department of Biological Sciences, Graduate School of Science, University of Tokyo Tokyo 113-0033 Japan., Oishi S; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8601 Japan bode@itbm.nagoya-u.ac.jp oishi@itbm.nagoya-u.ac.jp., Bode JW; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8601 Japan bode@itbm.nagoya-u.ac.jp oishi@itbm.nagoya-u.ac.jp.; Laboratorium für Organische Chemie, Department of Chemistry and Applied Biosciences, ETH Zürich Zürich 8093 Switzerland bode@org.chem.ethz.ch.
Jazyk:	angličtina
Zdroj:	RSC chemical biology [RSC Chem Biol] 2022 Mar 18; Vol. 3 (6), pp. 721-727. Date of Electronic Publication: 2022 Mar 18 (Print Publication: 2022).
DOI:	10.1039/d2cb00039c
Abstrakt:	The synthesis of secreted cysteine-rich proteins (CRPs) is a long-standing challenge due to protein aggregation and premature formation of inter- and intramolecular disulfide bonds. Chemical synthesis provides reduced CRPs with a higher purity, which is advantageous for folding and isolation. Herein, we report the chemical synthesis of pollen tube attractant CRPs Torenia fournieri LURE (TfLURE) and Torenia concolor LURE (TcLURE) and their chimeric analogues via α-ketoacid-hydroxylamine (KAHA) ligation. The bioactivity of chemically synthesized TfLURE protein was shown to be comparable to E. coli expressed recombinant protein through in vitro assay. The convergent protein synthesis approach is beneficial for preparing these small protein variants efficiently. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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