COVID-19 vaccination elicits an evolving, cross-reactive antibody response to epitopes conserved with endemic coronavirus spike proteins.

Autor:	Elko EA; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Nelson GA; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Mead HL; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Kelley EJ; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Carvalho ST; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Sarbo NG; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Harms CE; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Le Verche V; Center for Gene Therapy, Department of Hematology and Hematopoietic Cell Transplantation, City of Hope National Medical Center, Duarte, CA, USA., Cardoso AA; Center for Gene Therapy, Department of Hematology and Hematopoietic Cell Transplantation, City of Hope National Medical Center, Duarte, CA, USA., Ely JL; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Boyle AS; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Piña A; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Henson SN; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Rahee F; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA., Keim PS; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Celona KR; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Yi J; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Settles EW; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Bota DA; Alpha Stem Cell Clinic, University of California at Irvine, Irvine, CA, USA., Yu GC; George C. Yu, MD, Inc., Camarillo, CA, USA., Morris SR; Alpha Stem Cell Clinic, University of California at San Diego, La Jolla, CA, USA., Zaia JA; Center for Gene Therapy, Department of Hematology and Hematopoietic Cell Transplantation, City of Hope National Medical Center, Duarte, CA, USA., Ladner JT; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Altin JA; The Translational Genomics Research Institute (TGen), Flagstaff, AZ, USA. Electronic address: jaltin@tgen.org.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2022 Jul 05; Vol. 40 (1), pp. 111022. Date of Electronic Publication: 2022 Jun 13.
DOI:	10.1016/j.celrep.2022.111022
Abstrakt:	The COVID-19 pandemic has triggered the first widespread vaccination campaign against a coronavirus. Many vaccinated subjects are previously naive to SARS-CoV-2; however, almost all have previously encountered other coronaviruses (CoVs), and the role of this immunity in shaping the vaccine response remains uncharacterized. Here, we use longitudinal samples and highly multiplexed serology to identify mRNA-1273 vaccine-induced antibody responses against a range of CoV Spike epitopes, in both phylogenetically conserved and non-conserved regions. Whereas reactivity to SARS-CoV-2 epitopes shows a delayed but progressive increase following vaccination, we observe distinct kinetics for the endemic CoV homologs at conserved sites in Spike S2: these become detectable sooner and decay at later time points. Using homolog-specific antibody depletion and alanine-substitution experiments, we show that these distinct trajectories reflect an evolving cross-reactive response that can distinguish rare, polymorphic residues within these epitopes. Our results reveal mechanisms for the formation of antibodies with broad reactivity against CoVs. Competing Interests: Declaration of interests The authors declare that they have no competing interests. (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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