Rapid detection of bacteria using gold nanoparticles in SERS with three different capping agents: Thioglucose, polyvinylpyrrolidone, and citrate.

Autor: Deb M; School of Electrical Engineering and Computer Science, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada. Electronic address: mdeb091@uottawa.ca., Hunter R; Ottawa-Carleton Institute for Biomedical Engineering, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada., Taha M; The Ottawa Hospital Research Institute, Ottawa, Ontario K1Y 4E9, Canada., Abdelbary H; The Ottawa Hospital Research Institute, Ottawa, Ontario K1Y 4E9, Canada., Anis H; School of Electrical Engineering and Computer Science, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2022 Nov 05; Vol. 280, pp. 121533. Date of Electronic Publication: 2022 Jun 20.
DOI: 10.1016/j.saa.2022.121533
Abstrakt: The increase in outbreaks of emerging and re-emerging bacterial infections over the last few decades calls for their rapid detection and treatment. Surface-enhanced Raman spectroscopy (SERS) is a technique that can be applied to develop fast screening systems for bacterial presence in biological samples. Optimizing the capping agents in nanoparticle synthesis is important because capping agents are responsible for controlling the morphological features and chemical properties of the nanoparticles that are essential for SERS. To the best of our knowledge, this paper is the first to study the application of gold nanoparticles capped with thioglucose and polyvinylpyrrolidone (PVP) in SERS detection of bacteria as an alternative to the citrate-capped gold nanoparticles that are often used in SERS detection of bacteria. Three different species of bacteria were used in this study: Cutibacterium acnes, Escherichia coli and Staphylococcus aureus (methicillin-sensitive and methicillin-resistant). This study demonstrates that the thioglucose, citrate both show good contribution in bacterial species identification and the thioglucose shows the best among the three capping agents in two types of S. aureus identification. Moreover, although PVP showed high Raman peaks in the SERS spectrum for each type of bacteria, it showed least contribution in identifying species and strains due to its low efficacy in producing responses from different nucleic acid components in the bacteria cells.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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