Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level.

Autor: Wang F; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen, Qingdao, China.; Department of Biomedicine, Aarhus University, Aarhus, Denmark.; BGI-Shenzhen, Shenzhen, China., Ding P; BGI-Shenzhen, Shenzhen, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China., Liang X; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen, Qingdao, China.; Department of Biology, University of Copenhagen, Copenhagen, Denmark., Ding X; BGI-Shenzhen, Shenzhen, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China., Brandt CB; Department of Biomedicine, Aarhus University, Aarhus, Denmark.; Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark., Sjöstedt E; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden., Zhu J; BGI-Shenzhen, Shenzhen, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China., Bolund S; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden., Zhang L; BGI-Shenzhen, Shenzhen, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.; MGI, BGI-Shenzhen, Shenzhen, China., de Rooij LPMH; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Leuven, Belgium.; Department of Oncology, Leuven Cancer Institute, KU Leuven, Leuven, Belgium., Luo L; BGI-Shenzhen, Shenzhen, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China., Wei Y; BGI-Shenzhen, Shenzhen, China.; College of Basic Medicine, Qingdao University, Qingdao, China., Zhao W; BGI-Shenzhen, Shenzhen, China.; College of Basic Medicine, Qingdao University, Qingdao, China., Lv Z; BGI-Shenzhen, Shenzhen, China.; College of Basic Medicine, Qingdao University, Qingdao, China., Haskó J; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Li R; BGI-Shenzhen, Shenzhen, China.; College of Basic Medicine, Qingdao University, Qingdao, China., Qin Q; BGI-Shenzhen, Shenzhen, China.; College of Basic Medicine, Qingdao University, Qingdao, China., Jia Y; BGI-Shenzhen, Shenzhen, China.; College of Basic Medicine, Qingdao University, Qingdao, China., Wu W; BGI-Shenzhen, Shenzhen, China.; College of Basic Medicine, Qingdao University, Qingdao, China., Yuan Y; School of Basic Medical Sciences, Binzhou Medical University, Yantai, China., Pu M; BGI-Shenzhen, Shenzhen, China.; College of Basic Medicine, Qingdao University, Qingdao, China., Wang H; BGI-Shenzhen, Shenzhen, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China., Wu A; Institute of Systems Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.; Suzhou Institute of Systems Medicine, Suzhou, China., Xie L; MGI, BGI-Shenzhen, Shenzhen, China., Liu P; MGI, BGI-Shenzhen, Shenzhen, China., Chen F; MGI, BGI-Shenzhen, Shenzhen, China., Herold J; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Kalucka J; Department of Biomedicine, Aarhus University, Aarhus, Denmark.; Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark.; Aarhus University of Advanced Studies (AIAS), Aarhus University, Aarhus, Denmark., Karlsson M; Department of Protein Science, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden., Zhang X; BGI-Shenzhen, Shenzhen, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China., Helmig RB; Department of Obstetrics and Gynecology, Aarhus University Hospital, Aarhus, Denmark., Fagerberg L; Department of Protein Science, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden., Lindskog C; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Pontén F; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Uhlen M; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.; Department of Protein Science, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden., Bolund L; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen, Qingdao, China.; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Jessen N; Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark., Jiang H; MGI, BGI-Shenzhen, Shenzhen, China., Xu X; BGI-Shenzhen, Shenzhen, China., Yang H; BGI-Shenzhen, Shenzhen, China.; IBMC-BGI Center, the Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, China., Carmeliet P; Department of Biomedicine, Aarhus University, Aarhus, Denmark.; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Leuven, Belgium.; Department of Oncology, Leuven Cancer Institute, KU Leuven, Leuven, Belgium.; Center for Biotechnology, Khalifa University of Science and Technology, Abu Dhabi, United Arab Emirates., Mulder J; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden., Chen D; BGI-Shenzhen, Shenzhen, China. cds@ism.pumc.edu.cn.; Institute of Systems Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China. cds@ism.pumc.edu.cn.; Suzhou Institute of Systems Medicine, Suzhou, China. cds@ism.pumc.edu.cn., Lin L; Department of Biomedicine, Aarhus University, Aarhus, Denmark. lin.lin@biomed.au.dk.; Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark. lin.lin@biomed.au.dk., Luo Y; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen, Qingdao, China. alun@biomed.au.dk.; Department of Biomedicine, Aarhus University, Aarhus, Denmark. alun@biomed.au.dk.; BGI-Shenzhen, Shenzhen, China. alun@biomed.au.dk.; Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark. alun@biomed.au.dk.; IBMC-BGI Center, the Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, China. alun@biomed.au.dk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Jun 24; Vol. 13 (1), pp. 3620. Date of Electronic Publication: 2022 Jun 24.
DOI: 10.1038/s41467-022-31388-z
Abstrakt: Pigs are valuable large animal models for biomedical and genetic research, but insights into the tissue- and cell-type-specific transcriptome and heterogeneity remain limited. By leveraging single-cell RNA sequencing, we generate a multiple-organ single-cell transcriptomic map containing over 200,000 pig cells from 20 tissues/organs. We comprehensively characterize the heterogeneity of cells in tissues and identify 234 cell clusters, representing 58 major cell types. In-depth integrative analysis of endothelial cells reveals a high degree of heterogeneity. We identify several functionally distinct endothelial cell phenotypes, including an endothelial to mesenchymal transition subtype in adipose tissues. Intercellular communication analysis predicts tissue- and cell type-specific crosstalk between endothelial cells and other cell types through the VEGF, PDGF, TGF-β, and BMP pathways. Regulon analysis of single-cell transcriptome of microglia in pig and 12 other species further identifies MEF2C as an evolutionally conserved regulon in the microglia. Our work describes the landscape of single-cell transcriptomes within diverse pig organs and identifies the heterogeneity of endothelial cells and evolutionally conserved regulon in microglia.
(© 2022. The Author(s).)
Databáze: MEDLINE
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