Lipocalin 15 in the olfactory mucus is a biomarker for Bowman's gland activity.

Autor: Ijichi C; Food Products Division, Technology & Solution Development Center, Institute of Food Science and Technologies, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan. chiori_ijichi@ajinomoto.com., Kondo K; Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan. kondok-tky@umin.ac.jp., Kobayashi M; Department of Otorhinolaryngology-Head and Neck Surgery, Mie University Graduate School of Medicine, Tsu, Mie, Japan., Shirasawa A; Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan., Shimbo K; Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan., Nakata K; Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan., Maruyama Y; Food Products Division, Technology & Solution Development Center, Institute of Food Science and Technologies, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan., Ihara Y; Food Products Division, Technology & Solution Development Center, Institute of Food Science and Technologies, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan., Kawato Y; Food Products Division, Technology & Solution Development Center, Institute of Food Science and Technologies, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan., Mannen T; Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan., Takeshita R; Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan., Kikuchi Y; Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan., Saito Y; Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan., Yamasoba T; Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jun 24; Vol. 12 (1), pp. 9984. Date of Electronic Publication: 2022 Jun 24.
DOI: 10.1038/s41598-022-13464-y
Abstrakt: Olfactory mucus contributes to the specific functions of the olfactory mucosa, but the composition and source of mucus proteins have not been fully elucidated. In this study, we used comprehensive proteome analysis and identified lipocalin 15 (LCN15), a human-specific lipocalin family protein, as an abundant component of the olfactory mucus. Western blot analysis and enzyme-linked immunosorbent assay (ELISA) using a newly generated anti-LCN15 antibody showed that LCN15 was concentrated in olfactory mucus samples, but not in respiratory mucus samples. Immunohistochemical staining using anti-LCN15 antibody revealed that LCN15 localized to the cytokeratin 18-positive Bowman's glands of the olfactory cleft mucosa. Quantitative image analysis revealed that the area of LCN15 immunoreactivity along the olfactory cleft mucosa significantly correlated with the area of neuron-specific Protein-Gene Product 9.5 (PGP9.5) immunoreactivity, suggesting that LCN15 is produced in non-degenerated areas of the olfactory neuroepithelium. ELISA demonstrated that the concentration of LCN15 in the mucus was lower in participants with normal olfaction (≥ 50 years) and also tended to be lower in patients with idiopathic olfactory loss (≥ 50 years) than in participants with normal olfaction (< 50 years). Thus, LCN15 may serve as a biomarker for the activity of the Bowman's glands.
(© 2022. The Author(s).)
Databáze: MEDLINE
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