Detection of DNA adducts derived from the tobacco carcinogens, benzo[a]pyrene and dibenzo[def,p]chrysene in human oral buccal cells.

Autor:	Chen KM; Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA, USA., Sun YW; Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA, USA., Krebs NM; Department of Public Health Sciences, Pennsylvania State University College of Medicine, Hershey, PA, USA., Sun D; Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA, USA., Krzeminski J; Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA, USA., Reinhart L; Department of Public Health Sciences, Pennsylvania State University College of Medicine, Hershey, PA, USA., Gowda K; Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA, USA., Amin S; Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA, USA., Mallery S; College of Dentistry, Division of Oral Maxillofacial Pathology, Ohio State University, Columbus, OH, USA., Richie JP; Department of Public Health Sciences, Pennsylvania State University College of Medicine, Hershey, PA, USA., El-Bayoumy K; Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA, USA.
Jazyk:	angličtina
Zdroj:	Carcinogenesis [Carcinogenesis] 2022 Sep 19; Vol. 43 (8), pp. 746-753.
DOI:	10.1093/carcin/bgac058
Abstrakt:	Polycyclic aromatic hydrocarbons (PAHs) are recognized as potential etiological agents in the development of oral cancer in smokers. In particular, benzo[a]pyrene (B[a]P) and dibenzo[def,p]chrysene (DB[a,l]P) are detected in cigarette smoke and the environment and can induce DNA damage, mutagenesis and carcinogenesis in the oral cavity of rodents. Consequently, DNA adducts are regarded as the most direct markers of genotoxicity and can be used as biomarkers of cancer risk. Thus, this study used LC-MS/MS analysis with isotope labeled internal standard to detect and quantify DNA adducts derived from B[a]P and DB[a,l]P in buccal cells of cigarette smokers and non-smokers. Participants in this study include 21 smokers and 16 non-smokers. Our data are the first to report that levels (mean ± SD) of BPDE-N2-dG were significantly (P < 0.001) higher in smokers (20.18 ± 8.40 adducts/108 dG) than in non-smokers (0.84 ± 1.02 adducts/108 dG). Likewise, levels of DBPDE-N6-dA in smokers (5.49 ± 3.41 adducts/108 dA) were significantly higher (P = 0.019) than non-smokers (2.76 ± 2.29 adducts/108 dA). Collectively, the results of this clinical study support that PAHs in tobacco smoke can contribute to the development of oral cancer in humans. (© The Author(s) 2022. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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