Iron Oxide Nanoparticles Functionalized with Fucoidan: a Potential Theranostic Nanotool for Hepatocellular Carcinoma.

Autor: Geinguenaud F; Université Sorbonne Paris Nord and Université Paris Cité, INSERM, LVTS, 75018, Paris, France., Sainte-Catherine O; Université Sorbonne Paris Nord and Université Paris Cité, INSERM, LVTS, 75018, Paris, France., Poirier F; Université Sorbonne Paris Nord, Plateforme PPUP13, 93000, Bobigny, France., Besnard V; Université Sorbonne Paris Nord, H&P INSERM, UMR 1272, 93000, Bobigny, France., Haddad O; Université Sorbonne Paris Nord and Université Paris Cité, INSERM, LVTS, 75018, Paris, France., Chaubet F; Université Sorbonne Paris Nord and Université Paris Cité, INSERM, LVTS, 75018, Paris, France., Lalatonne Y; Université Sorbonne Paris Nord and Université Paris Cité, INSERM, LVTS, 75018, Paris, France.; Service de Biophysique et Médecine Nucléaire, AP-HP, Hôpital Avicenne, 9300, Bobigny, France., Lutomski D; Université Sorbonne Paris Nord, Plateforme PPUP13, 93000, Bobigny, France., Sutton A; Université Sorbonne Paris Nord and Université Paris Cité, INSERM, LVTS, 75018, Paris, France.; Service de Biochimie, AP-HP, Hôpital Jean Verdier, 93140, Bondy, France., Motte L; Université Sorbonne Paris Nord and Université Paris Cité, INSERM, LVTS, 75018, Paris, France.
Jazyk: angličtina
Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2022 Aug 17; Vol. 23 (16), pp. e202200265. Date of Electronic Publication: 2022 Jul 11.
DOI: 10.1002/cbic.202200265
Abstrakt: Fucoidan is a natural sulfated polysaccharide with a large range of biological activities including anticancer and anti-oxidation activities. Hepatocellular carcinoma is the fourth most common aggressive cancer type. The aim of this study was to investigate the bioactivity of free fucoidan versus its vectorization using nanoparticles (NPs) in human hepatoma cells, Huh-7. Iron oxide NPs were functionalized with fucoidan by a one-step surface complexation. NP cellular uptake was quantified by magnetic measurement at various extracellular iron concentrations. Cell invasion and migration were reduced with NPs while free fucoidan increases these events at low fucoidan concentration (≤0.5 μM). Concomitantly, a high decrease of reactive oxygen species production related with a decrease of the matrix metalloproteinase-9 activity and an increase of its expression was observed with NPs compared to free fucoidan. A proteomic analysis evidenced that some fucoidan regulated proteins appeared, which were related to protein synthesis, N-glycan processing, and cellular stress. To our knowledge, this is the first study which reveals such activity induced by fucoidan. These results pave the way for USPIO-fucoidan-NPs as potential theranostic nanotools for hepatocellular carcinoma treatment.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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