| Autor: |
da Costa Lopes J; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Falcão LFM; Department of Pathology, State University of Para, Belem 66050-540, Brazil., Martins Filho AJ; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Carvalho MLG; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Mendes CCH; Department of Pathology, State University of Para, Belem 66050-540, Brazil., Olímpio FA; Tripical Medicine Unit, Federal University of Para, Belem 66075-110, Brazil., do Socorro Cabral Miranda V; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Dos Santos LC; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Chiang JO; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Cruz ACR; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Galúcio VCA; Department of Pathology, State University of Para, Belem 66050-540, Brazil., do Socorro da Silva Azevedo R; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Martins LC; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil., Duarte MIS; Schhol of Medicine, Sao Paulo University, Sao Paulo 05508-070, Brazil., de Sousa JR; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil.; Department of Pathology, State University of Para, Belem 66050-540, Brazil., da Costa Vasconcelos PF; Evandro Chagas Institut, Ministry of Health, Ananindeua 67015-120, Brazil.; Department of Pathology, State University of Para, Belem 66050-540, Brazil., Quaresma JAS; Department of Pathology, State University of Para, Belem 66050-540, Brazil.; Tripical Medicine Unit, Federal University of Para, Belem 66075-110, Brazil.; Schhol of Medicine, Sao Paulo University, Sao Paulo 05508-070, Brazil. |
| Abstrakt: |
Yellow fever (YF), a non-contagious infectious disease, is endemic or enzootic to the tropical regions of the Americas and Africa. Periodic outbreaks or epidemics have a significant impact on public health. Programmed cell death, or apoptosis, is generally characterised by distinct morphological changes and energy-dependent biochemical pathways. In this study, we performed immunohistochemistry analysis to identify and quantify proteases and protein targets involved in the cascade that triggers apoptosis in YF virus (YFV)-infected human hepatocytes. Liver tissue samples were collected from 26 individuals, among whom 21 were diagnosed as YF-positive, and five were flavivirus-negative and died due to other causes. The histopathological alterations in YFV-positive cases were characterised by the presence of apoptotic bodies, steatosis, cellular swelling, and extensive necrosis and haemorrhage in the hepatic lobules. Additionally, we observed an abundance of inflammatory infiltrates in the portal tract. The expression of various apoptotic markers in the hepatic parenchyma, including CASPASE 3, CASPASE 8, BAX, FAS, FASL, GRANZYME B, and SURVIVIN, differed between YFV-positive cases and controls. Collectively, this study confirmed the complexity of YFV infection-induced apoptosis in situ. However, our data suggest that apoptosis in liver parenchyma lesions may significantly contribute to the pathogenesis of fatal YF in humans. |
