| Autor: |
Bergami F; Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Arena F; Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Pattonieri EF; Nephrology, Dialysis and Transplantation Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Gregorini M; Nephrology, Dialysis and Transplantation Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Meloni F; Department of Internal Medicine and Medical Therapeutics, University of Pavia, 27100 Pavia, Italy., Abelli M; General Surgery 4 Unit-Abdominal Transplantation, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Ticozzelli E; General Surgery 4 Unit-Abdominal Transplantation, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Testa G; Pediatrics Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Lilleri D; Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Cassaniti I; Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Baldanti F; Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.; Department of Clinical, Surgical, Diagnostics and Pediatric Sciences, University of Pavia, 27100 Pavia, Italy. |
| Abstrakt: |
Since the identification of the new severe acute respiratory syndrome virus 2 (SARS-CoV-2), a huge effort in terms of diagnostic strategies has been deployed. To date, serological assays represent a valuable tool for the identification of recovered COVID-19 patients and for the monitoring of immune response elicited by vaccination. However, the role of T-cell response should be better clarified and simple and easy to perform assays should be routinely introduced. The main aim of this study was to compare a home-made assay for whole blood stimulation with a standardized ELISpot assay design in our laboratory for the assessment of spike-specific T-cell response in vaccinated subjects. Even if a good correlation between the assays was reported, a higher percentage of responder subjects was reported for immunocompromised subjects with ELISpot assay (56%) than home-made whole blood stimulation assay (33%). Additionally, three commercial assays were compared with our home-made assay, reporting a good agreement in terms of both positive and negative results. |
