A mechanistic understanding of polyethylene biodegradation by the marine bacterium Alcanivorax.

Autor: Zadjelovic V; School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK. Electronic address: Vinko.Zadjelovic-Varas@warwick.ac.uk., Erni-Cassola G; School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK; Program Man-Society-Environment (MGU), University of Basel, 4051 Basel, Switzerland., Obrador-Viel T; Department of Biology, University of the Balearic Islands, Palma 07122, Spain., Lester D; Polymer Characterisation Research Technology Platform, University of Warwick, Coventry CV4 7AL, UK., Eley Y; School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston B15 2TT, UK., Gibson MI; Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK., Dorador C; Laboratorio de Complejidad Microbiana y Ecología Funcional, Instituto Antofagasta, Universidad de Antofagasta, Chile; Departamento de Biotecnología, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta Angamos 601, Antofagasta, Chile; Centre for Biotechnology & Bioengineering (CeBiB) Santiago, Chile., Golyshin PN; Centre for Environmental Biotechnology, School of Natural Sciences, Bangor University, Bangor LL57 2UW, UK., Black S; Department of Geography and Environmental Science, University of Reading, UK., Wellington EMH; School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK., Christie-Oleza JA; School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK; Department of Biology, University of the Balearic Islands, Palma 07122, Spain. Electronic address: joseph.christie@uib.eu.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2022 Aug 15; Vol. 436, pp. 129278. Date of Electronic Publication: 2022 Jun 03.
DOI: 10.1016/j.jhazmat.2022.129278
Abstrakt: Polyethylene (PE) is one of the most recalcitrant carbon-based synthetic materials produced and, currently, the most ubiquitous plastic pollutant found in nature. Over time, combined abiotic and biotic processes are thought to eventually breakdown PE. Despite limited evidence of biological PE degradation and speculation that hydrocarbon-degrading bacteria found within the plastisphere is an indication of biodegradation, there is no clear mechanistic understanding of the process. Here, using high-throughput proteomics, we investigated the molecular processes that take place in the hydrocarbon-degrading marine bacterium Alcanivorax sp. 24 when grown in the presence of low density PE (LDPE). As well as efficiently utilising and assimilating the leachate of weathered LDPE, the bacterium was able to reduce the molecular weight distribution (M w from 122 to 83 kg/mol) and overall mass of pristine LDPE films (0.9 % after 34 days of incubation). Most interestingly, Alcanivorax acquired the isotopic signature of the pristine plastic and induced an extensive array of metabolic pathways for aliphatic compound degradation. Presumably, the primary biodegradation of LDPE by Alcanivorax sp. 24 is possible via the production of extracellular reactive oxygen species as observed both by the material's surface oxidation and the measurement of superoxide in the culture with LDPE. Our findings confirm that hydrocarbon-biodegrading bacteria within the plastisphere may in fact have a role in degrading PE.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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