| Autor: |
Badr H; Bacteriology Unit, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Ministry of Agriculture, Agricultural Research Center (ARC), Nadi El-Seid Street, Dokki, P.O. Box 246, Giza 12618, Egypt., Samir A; Biotechnology Unit, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Ministry of Agriculture, Agricultural Research Center (ARC), Nadi El-Seid Street, Dokki, P.O. Box 246, Giza 12618, Egypt., El-Tokhi EI; Biotechnology Department, Animal Health Research Institute, Agricultural Research Center (ARC), Giza 12618, Egypt., Shahein MA; Department of Virology Research, Animal Health Research Institute, Agricultural Research Center (ARC), Giza 12618, Egypt., Rady FM; Mycology Department, Animal Health Research Institute, Agricultural Research Center (ARC), Shebin Elkom 32511, Egypt., Hakim AS; Microbiology and Immunology Department, National Research Centre, 33 Bohouth st., Dokki, Cairo 12622, Egypt., Fouad EA; Microbiology and Immunology Department, National Research Centre, 33 Bohouth st., Dokki, Cairo 12622, Egypt., El-Sady EF; Microbiology and Immunology Department, National Research Centre, 33 Bohouth st., Dokki, Cairo 12622, Egypt., Ali SF; Bacteriology Department, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, Egypt. |
| Abstrakt: |
Chickens continue to be an important reservoir of zoonotic multidrug-resistant illnesses. Antimicrobial resistance correlated with colistin has emerged as a critical concern worldwide in the veterinary field and the public health sector. The current study investigated the prevalence of multidrug-resistant avian pathogenic Escherichia coli among chicken farms in three Egyptian governorates, focusing on colistin resistance assessment. A total of 56 Escherichia coli isolates were recovered out of 120 pooled samples obtained from diseased chicken broilers (46.7%). The E. coli isolates were serotyped to nine different serotypes; the highest incidence was for O125 ( n = 18). The E. coli isolates demonstrated multidrug-resistant patterns against 10 antibiotics, especially clindamycin, tetracycline, streptomycin and ampicillin, by 100, 100, 96.4 and 92.9%, respectively. On the other hand, colistin resistance was 41.1% using AST. All E. coli isolates displayed positive colistin resistance growth on chromogenic medium, but only 25% represented this positivity via MIC estimation and Sensititre kit. PCR results revealed that all isolates harbored mcr -1, but no isolates harbored the other 2-5 mcr genes. In conclusion, the study demonstrated the emergence of multidrug-resistant, especially colistin-resistant, E. coli among chicken broiler flocks, and mcr -1 is the master gene of the colistin resistance feature. |
