The antiproliferative and apoptotic potential of Clinacanthus nutans against human breast cancer cells through targeted apoptosis pathway.

Autor: Ismail NZ; Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Penang, Kepala Batas, Malaysia., Md Saad S; Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Penang, Kepala Batas, Malaysia., Adebayo IA; Department of Clinical Biology, School of Medicine and Pharmacy, College of Medicine and Health Sciences, University of Rwanda, Kigali, Rwanda.; Analystical Biochemistry Research Centre, Universiti Sains Malaysia, Penang, Malaysia.; Microbiology and Immunology Department, School of Biomedical Sciences, Kampala International University, Western Campus, P.O. Box 71, Ishaka-Bushenyi, Uganda., Md Toha Z; Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Penang, Kepala Batas, Malaysia., Abas R; Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Penang, Kepala Batas, Malaysia., Mohamad Zain NN; Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Penang, Kepala Batas, Malaysia., Arsad H; Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Penang, Kepala Batas, Malaysia. hasniarsad@usm.my.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2022 Nov; Vol. 29 (54), pp. 81685-81702. Date of Electronic Publication: 2022 Jun 23.
DOI: 10.1007/s11356-022-20858-y
Abstrakt: Clinacanthus nutans dichloromethane fraction (CN-Dcm) extract has previously been proven to suppress breast cancer (MCF7) cell proliferation. Despite this, the extrinsic and intrinsic apoptosis mechanisms involved in C. nutans extract-treated MCF7 cells are still unknown. This study was intended to subfractionate CN-Dcm extract using column chromatography and analyse the treated MCF7 cells using the CellTiter 96® AQueous One Solution Cell Proliferation (MTS) assay, Annexin V/propidium iodide (PI) assay, western blot, and reverse transcription-qualitative polymerase chain reaction (RT-qPCR). Out of nine subfraction extracts (SF1 to SF9), SF2 extract strongly inhibited MCF7 cells with the lowest IC 50 value (23.51 ± 1.00 µg/mL) and substantially induced apoptosis in the MCF7 cells. In treated MCF7 cells, SF2 extract significantly upregulated the expression of P53, BAX, BID, caspase-8, caspase-9, and caspase-3, while downregulating the expression of BCL2. The presence of potential bioactive chemical compounds in the SF2 extract was identified using liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Thus, the SF2 extract has the potential to induce apoptosis in MCF7 cells through intrinsic and extrinsic pathways.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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