Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags.
| Autor: | Sultonova M; Department of Biology, University of Prince Edward Island, Charlottetown, PE, Canada., Pinto DM; National Research Council, Halifax, NS, Canada., Murphy JP; Department of Biology, University of Prince Edward Island, Charlottetown, PE, Canada. jpmurphy@upei.ca. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2508, pp. 211-223. |
| DOI: | 10.1007/978-1-0716-2376-3_16 |
| Abstrakt: | Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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