The effect of discrete wavelengths of visible light on the developing murine embryo.

Autor: Campugan CA; School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia., Lim M; School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia., Chow DJX; School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia., Tan TCY; School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia., Li T; School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia., Saini AA; School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia., Orth A; National Research Council of Canada, Ottawa, Ontario, Canada., Reineck P; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, School of Science, Royal Melbourne Institute of Technology, Melbourne, VIC, 3000, Australia., Schartner EP; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia.; School of Physical Sciences, The University of Adelaide, Adelaide, SA, 5005, Australia., Thompson JG; School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia.; Fertilis Pty Ltd, Adelaide, South Australia, 5005, Australia., Dholakia K; School of Physics and Astronomy, University of St Andrews, North Haugh, Scotland , KY16 9SS, UK.; School of Biological Sciences, The University of Adelaide, Adelaide, SA, 5005, Australia.; Department of Physics, College of Science, Yonsei University, Seoul, 03722, South Korea., Dunning KR; School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia. kylie.dunning@adelaide.edu.au.; Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia. kylie.dunning@adelaide.edu.au.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia. kylie.dunning@adelaide.edu.au.
Jazyk: angličtina
Zdroj: Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2022 Aug; Vol. 39 (8), pp. 1825-1837. Date of Electronic Publication: 2022 Jun 23.
DOI: 10.1007/s10815-022-02555-4
Abstrakt: Purpose: A current focus of the IVF field is non-invasive imaging of the embryo to quantify developmental potential. Such approaches use varying wavelengths to gain maximum biological information. The impact of irradiating the developing embryo with discrete wavelengths of light is not fully understood. Here, we assess the impact of a range of wavelengths on the developing embryo.
Methods: Murine preimplantation embryos were exposed daily to wavelengths within the blue, green, yellow, and red spectral bands and compared to an unexposed control group. Development to blastocyst, DNA damage, and cell number/allocation to blastocyst cell lineages were assessed. For the longer wavelengths (yellow and red), pregnancy/fetal outcomes and the abundance of intracellular lipid were investigated.
Results: Significantly fewer embryos developed to the blastocyst stage when exposed to the yellow wavelength. Elevated DNA damage was observed within embryos exposed to blue, green, or red wavelengths. There was no effect on blastocyst cell number/lineage allocation for all wavelengths except red, where there was a significant decrease in total cell number. Pregnancy rate was significantly reduced when embryos were irradiated with the red wavelength. Weight at weaning was significantly higher when embryos were exposed to yellow or red wavelengths. Lipid abundance was significantly elevated following exposure to the yellow wavelength.
Conclusion: Our results demonstrate that the impact of light is wavelength-specific, with longer wavelengths also impacting the embryo. We also show that effects are energy-dependent. This data shows that damage is multifaceted and developmental rate alone may not fully reflect the impact of light exposure.
(© 2022. The Author(s).)
Databáze: MEDLINE
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