Optimization through a Box-Behnken Experimental Design of the Microwave-Assisted Extraction of the Psychoactive Compounds in Hallucinogenic Fungi ( Psylocibe cubensis ).

Autor: Polo-Castellano C; Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, 11510 Puerto Real, Spain., Álvarez JÁ; Department of Physical Chemistry, Faculty of Sciences, INBIO, University of Cadiz, 11510 Puerto Real, Spain., Palma M; Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, 11510 Puerto Real, Spain., Barbero GF; Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, 11510 Puerto Real, Spain., Ayuso J; Department of Physical Chemistry, Faculty of Sciences, INBIO, University of Cadiz, 11510 Puerto Real, Spain., Ferreiro-González M; Department of Physical Chemistry, Faculty of Sciences, INBIO, University of Cadiz, 11510 Puerto Real, Spain.
Jazyk: angličtina
Zdroj: Journal of fungi (Basel, Switzerland) [J Fungi (Basel)] 2022 Jun 02; Vol. 8 (6). Date of Electronic Publication: 2022 Jun 02.
DOI: 10.3390/jof8060598
Abstrakt: Hallucinogenic fungi, mainly those from the Psilocybe genus, are being increasingly consumed even though there is no control on their culture conditions. Due to the therapeutic potential as antidepressants and anxiolytics of the alkaloids that they produce (psilocin and psilocybin), some form of control on their production would be highly recommended. Prior to identifying their optimal culture condition, a methodology that allows their study is required. Microwave-assisted extraction method (MAE) is a technique that has proven its efficiency to extract different compounds from solid matrices. For this reason, this study intends to optimize a MAE method to extract the alkaloids found in Psylocibe cubensis . A surface-response Box-Behnken design has been employed to optimize such extraction method and significantly reduce time and other resources in the extraction process. Based on the Box-Behnken design, 50 °C temperature, 60% methanol as extraction solvent, 0.6 g:10 mL sample mass:solvent ratio and 5 min extraction time, were established as optimal conditions. These mild conditions, combined with a rapid and efficient UHPLC analysis result in a practical and economical methodology for the extraction of psilocin and psilocybin from Psylocibe cubensis .
Databáze: MEDLINE
Nepřihlášeným uživatelům se plný text nezobrazuje